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  1. 2xHNG Buffer
  2. 3T3-L1 Adipocyte Fractionation
  3. 3T3-L1 Plasma Membrane Isolation
  4. 4EBP1/2 Genotyping
  5. 5' RACE
  6. 50X TAE Buffer
  7. ACSF Media
  8. Acetate Incorporation into Lipid
  9. Acid Etching Coverslips
  10. Acrylamide Solution
  11. Actinomycin D
  12. Adding New Personnel to Animal Protocol
  13. Adipocyte Cell Counting with ImageJ
  14. Adipose Tissue Nuclear Isolation
  15. Affinity Purification of Proteins - FPLC
  16. Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
  17. Amitryptyline Treatment of Mice
  18. Amylose pull-down of glycogen-bound proteins
  19. Analysing Data on Armis or Great Lakes using Rmd Files
  20. Analysing Sequencing Results with SeqMan
  21. Analysis of FRET Images
  22. Antisense Oligo Knockdown in Mice
  23. Assessing isoproterenol-stimulated whole-body lipolysis in vivo
  24. BDNF PCR
  25. BHB Assay
  26. Back-Crossing of Mouse Strains
  27. Bradford Assay
  28. CAP Genotyping
  29. CHAPS Lysis Buffer
  30. CPY Assay
  31. Cesium Chloride Preparation of DNA
  32. ChIP Elution Buffer
  33. Check Clones for Correct Mutation
  34. Chemical Hygiene Plan
  35. Chromatin Immunoprecipitation
  36. Chromatin Immunoprecipitation for Tissue Samples
  37. Cloning CRISPR-Cas Plasmids
  38. Collecting and Storing Mouse Serum
  39. Colocalization
  40. Colony PCR
  41. Connect to Network Drives
  42. Cre Genotyping
  43. Cre PCR
  44. Creating New R Packages
  45. Culturing MIN6 Cells
  46. Culturing RAW 264.7 Cells
  47. Culturing S2 Cells
  48. Culturing and Differentiating C2C12 Cells
  49. Cycloheximide
  50. DNA Preparation from Tail Clip
  51. Data Management
  52. Deparaffinizing Histology Slides
  53. Designing Clones Using Benchling
  54. Designing and Generating CRISPR-Cas Mutants
  55. Designing and Preparing dsRNA
  56. Designing siRNA for Knockdown
  57. Determining Cortisol Levels in Human Hair
  58. Determining Mitochondrial Copy Number
  59. Determining Percent Purity
  60. Dexamethasone Treatment of Mice
  61. Differentiation of 3T3-L1 Cells
  62. Differentiation of MEFs
  63. Dilution Buffer
  64. Dilution Formula
  65. DsRNA Mediated Knockdown of S2 Cells
  66. Electroporation of 3T3-L1 Adipocytes
  67. Extraction of DNA from TRIZOL preparations
  68. FDB Muscle Dissection from Mice
  69. FM4-64 Labeling of Yeast Cells
  70. Farnham Lysis Buffer
  71. Finding and Ordering BAC Clones
  72. First Strand cDNA Synthesis (AB Kit)
  73. Fly Food Protocol
  74. Freezing and Thawing S2 Cells
  75. French Press
  76. Fugene Transfection of 293T/COS Cells
  77. GDF15 Genotyping
  78. GST-EEA1 Pulldown from Yeast
  79. GST-GTPase Pull Down Assay
  80. GST Pulldown Assay
  81. General Statistics
  82. Generating DMSO Stocks for Cell Culture
  83. Generating and Amplifying Adenoviral Constructs
  84. Generating and Displaying an Electostatic Surface
  85. Generation of Figures in Illustrator
  86. Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap)
  87. Genotyping Program
  88. Glucose Incorporation into Lipid and Glycogen
  89. Glucose Tolerance Test
  90. Glucose Uptake Assay
  91. Glycogen Determination from Cells
  92. Glycogen Determination from Tissues
  93. Glycogen Phosphorylase Assay
  94. Glycogen Synthase Assay
  95. GoTaq PCR Genotyping
  96. H&E Staining
  97. H&E Staining of Mouse Tissue
  98. HNTG Buffer
  99. HPLC - Designing a Method for a Sample or Series
  100. HPLC - Plumbing Setup
  101. HPLC - Running a Sample or Series
  102. Harvesting RNA from Cells grown in monolayer
  103. High Fat Diet Treatment
  104. High Salt Immune Complex Wash Buffer
  105. How to Find a Journal Article
  106. How to design primer pairs for determining the presence of CrispR-cas induced mutations
  107. Immobilizing a Protein on a CM5 Chip for SPR
  108. Immunofluoresence
  109. Immunoprecipitation
  110. Induction Conditions
  111. Inositol Labeling and Lipid Extraction
  112. Inositol Labelling of Yeast Cells
  113. Installing Software to a UNIX Server
  114. Insulin Tolerance Test
  115. Isolation of Mouse Embryonic Fibroblasts (MEFs)
  116. Isolation of Single Fibers for Seahorse Assays
  117. KRBH Buffer
  118. Ketone Body Tolerance Test
  119. LiCl Immune Complex Wash Buffer
  120. Lipid Transfection
  121. Lipofectamine Mediated Knockdown
  122. Lipofectamine Plasmid Transfection
  123. Lipofectamine Transfection of C2C12 Cells
  124. Lipolysis Assay from Primary Adipocytes
  125. Liposome Binding Assay
  126. Locating ChIP-seq peaks from ENCODE
  127. Low Salt Immune Complex Wash Buffer
  128. Luciferase Assay
  129. MTM1 Genotyping
  130. MTORC1 Kinase Assay
  131. Main Page
  132. Maintanence of 18C Fly Stocks
  133. Maintenance of 18C Fly Stocks
  134. Maintenance of Fly Stocks
  135. Making LB Agar Plates
  136. Mammary Gland Fat and SVF Separation
  137. Maternal Particulate Exposure Breeding
  138. Matrigel Basement Membrane Matrix
  139. Mc4r-Cone Genotyping
  140. Measurement of Glycolysis and Respiration Using Seahorse
  141. Measuring Autophagy in Tissue Culture Cells
  142. Measuring Cross-Sectional Area (CSA)
  143. Measuring Fasting Insulin
  144. Measuring Grip Strength
  145. Measuring Lifespan in Drosophila
  146. Measuring Phagocytosis using pHrodo Bioparticles
  147. Milk Creamatocrit
  148. Monitoring Food Intake
  149. Mutagenesis
  150. NADH NBT Staining
  151. Nr3c1 Genotyping
  152. Ob/ob Genotyping
  153. Oil red o stain for in vitro adipogenesis
  154. Ordering Barcodes for Mice
  155. Ordering Mice
  156. Ordering Prescriptions-University of Michigan
  157. Osmotic Minipump Implantation
  158. PAS Staining
  159. PCR Amplification of DNA
  160. PCR Amplification of DNA (KOD Polymerase)
  161. PCR Amplification with Platinum Taq High Fidelity
  162. PCR Analysis of Tail DNA
  163. PEI Mediated Plasmid Transfection
  164. Paraffin Embedding of Tissue Samples
  165. Parsing XSQ Files
  166. Performing Drosophila Crosses
  167. Perfusion of Mouse
  168. Phosphate Buffered Saline
  169. Phred Quality Scores
  170. Ppp1r3c Genotyping
  171. Preparation of Chloroquine Stocks
  172. Preparation of DIG Labelled Probes
  173. Preparation of Mouse Muscle for Cryosectioning
  174. Preparation of Orthovanadate Stocks
  175. Preparation of PEI Stocks
  176. Preparation of Protein Lysates from Cells
  177. Preparation of Protein Lysates from Mouse Tissues
  178. Preparation of RNA Sample from Cell Culture
  179. Preparation of RNA Samples from Mouse Tissues
  180. Preparation of Tail Samples (for Genotyping)
  181. Preparing Cell Lysates
  182. Preparing a SDS-PAGE Gel
  183. Preparing an Adenoviral shRNA Clone
  184. Preparing an Agarose Gel
  185. Preparing and Sending Tissues to Histology Core
  186. Primary Adipocyte Isolation
  187. Primer Design for qPCR
  188. Production of LentiCRISPR Viruses
  189. Protein-Lipid Overlay Assay
  190. Purification of GST-HA-S6K
  191. Purification of GST Fusion Proteins
  192. Purification of GST Fusion Proteins by GSTrap
  193. Purification of MBP Fusion Proteins
  194. Purification of miRNA and mRNA with TRIzol
  195. Pyruvate Tolerance Test
  196. QPCR
  197. QPCR - Lin Lab
  198. QPCR Analysis on ThermoCloud
  199. Quantification by Absorbance at 280nm
  200. Quantification of Vacuolar Colocalization
  201. Quantification of miRNA by SYBR Green qPCR
  202. Quantifying Relative Expression from qRT-PCR
  203. RIPA Buffer
  204. RNA-seq Pipeline for Known Transcripts
  205. RT-PCR primer design for ChIP
  206. Rab5 FRET Assay
  207. Rapamycin Injections
  208. Rapamycin Sensitivity Assay of Yeast
  209. Rapamycin Stock Solution
  210. Rapid Isolation of Genomic DNA from Yeast
  211. Rate of drop analysis
  212. Real Time PCR From Cell Culture
  213. Restriction Enzyme Based Cloning
  214. Restriction Enzyme Based Cloning - Ordering Primers
  215. SDS-PAGE Running Buffer
  216. SDS-PAGE Separating Gel Buffer
  217. SDS-PAGE Stacking Gel Buffer
  218. SHROB Genotyping
  219. SOP- Chloroform
  220. SOP - Animal Anesthetics
  221. SOP - Autoclave
  222. SOP - B-mercaptoethanol
  223. SOP - BVT.2733
  224. SOP - Biosafety Cabinets
  225. SOP - Bleach
  226. SOP - Bunsen Burners
  227. SOP - Carcinogens
  228. SOP - Centrifuge Safety
  229. SOP - Chloroform
  230. SOP - Compressed Gases
  231. SOP - Corrosives
  232. SOP - Cryogenic Materials
  233. SOP - Cytotoxins and Antineoplastic Drugs
  234. SOP - Electrophoresis
  235. SOP - Ethidium Bromide
  236. SOP - Flammables and Combustibles
  237. SOP - Formalin and Paraformaldehyde
  238. SOP - Irritants
  239. SOP - Needle Capping
  240. SOP - Phenol
  241. SOP - Rapamycin
  242. SOP - Sensitizers
  243. SOP - Tamoxifen
  244. SOP - Vacuum Pumps
  245. SREBF1 Genotyping Protocol
  246. SRY Genotyping
  247. Safety and Animal Training
  248. Scanning and Analyzing Western Blots Using LiCor Odyssey
  249. Seahorse - Mitochondrial Stress Test
  250. Seahorse XF Media
  251. Sending Samples for DNA Sequencing
  252. Sequence Alignment for Phylogenetic Analysis
  253. Serum Total Cholesterol Assay
  254. Set up qPCR Run on Roche LightCycler
  255. Set up qPCR Run on Thermo Cloud and QuantStudio
  256. Single fiber dissociation of intact rodent muscles
  257. Spill Response Procedures for Infectious agents and Recombinant DNA
  258. Splitting Cells
  259. Statistical Analysis
  260. Submitting Plasmids for Sequencing
  261. Surface Plasmon Resonance - Protein Lipid Interactions
  262. Surveyor Assay for Genomic DNA Mutations
  263. TAP Purification of Yeast Extracts
  264. TBST
  265. TC10b Genotyping
  266. TE Buffer
  267. TOPO Cloning
  268. TORC1 Kinase Buffer
  269. TSC-floxed Genotyping
  270. Tamoxifen Injections for Inducible Knockouts
  271. TaqMan SNP Array
  272. TaqMan SNP Assay
  273. TaqMan miRNA Assay
  274. Taqman
  275. Thawing Culture Cells
  276. Transfer Buffer
  277. Transformation of Bacteria
  278. Triglyceride Assay from Cells and Tissues
  279. Triglyceride Assay from Drosophila (German Method)
  280. Triglyceride Assay from Tissue Culture Cells
  281. Using Bioconductor To Analyse Beadarray Data
  282. Using Bioconductor To Analyse Beadarray Data (lumi)
  283. Using Bioconductor To Analyse Microarray Data
  284. Using GSEA for Gene Set Analyses of Transcriptional Data
  285. Using Git
  286. Using Github and Git for Code
  287. Using ImageJ to Quantify Bands
  288. Using Mr Bayes to For Phlyogenetic Analysis
  289. Using South To Migrate a Django Database
  290. Using the MBNI Cluster
  291. Using the echoMRI
  292. Using the pH Meter
  293. Verifying Kan Deletion Mutants from Genomic DNA
  294. Vinexin Genotyping
  295. Weigh-Suckle-Weigh Milk Volume Measurement
  296. Western Blotting
  297. Wiki new protocols
  298. Writing a Paper and Uploading Code to Github Repository
  299. YPD Media and Agar
  300. Yeast Invertase Assay
  301. Yeast Sch9 Phosphorylation Assay
  302. Yeast Selective Media and Agar
  303. Yeast Transfection (Small Scale)
  304. Yeast Transformation

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