Maternal Particulate Exposure Breeding

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  • Order C57BL6 mice at 42 days of age from
  • Once the mice arrive, place mice on breeder's chow for seven days
  • At 49 days of age, mate female and male mice.


  • On day 10 and 17 of gestation expose the mice to particulate or control (saline/cabosil).
    • Should be close to days 59 and 66 days of age.


  • Keep the mother's on breeder's chow through weaning.