Maternal Particulate Exposure Breeding
From Bridges Lab Protocols
- Order C57BL6 mice at 42 days of age from jaxmice.org
- Once the mice arrive, place mice on breeder's chow for seven days
- At 49 days of age, mate female and male mice.
- On day 10 and 17 of gestation expose the mice to particulate or control (saline/cabosil).
- Should be close to days 59 and 66 days of age.
- Keep the mother's on breeder's chow through weaning.