Ppp1r3c Genotyping

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PRIMERS

  • Fwd: TGCTCTCCTTTTCTCCACGT
  • Rev: CCGGACCTGAACCTTCTTCT
  • Neo Rev: GCAGCGCATCGCCTTCTATC

Make primers at 1uM (Fwd + Rev and Fwd + Neo Rev}. PCR primers separately.

PCR PROTOCOL

PCR Step Temperature Time Cycles Notes
1. Initiation/Melting 95 3:00 1
2. Denaturation 95 0:30 35
3. Annealing 60 1:00 35
4. Elongation 72 1:00 35 steps 2-4 to be run in sequence
5. Amplification 72 5:00 1
6. Finish 4 infinite n/a