GoTaq PCR Genotyping

Materials

• GoTaq Master Mix
• Primer Mix (from 100uM Primer Stocks): Add 10uL of forward and reverse primer, 20uL total, into 980uL of dH20 to make a 1uM solution.
• Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 15uL of template. If doing several samples make a master mix of primer mix + template (25 uL GOTaq + 10 uL Primer Mix)x # of Samples + 1
• Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr.

---

Procedure

• Prepare gel 30 minutes before PCR is finished.
• Prepare Reaction Mixture, adding 13x all materials except for the template.
• Add this mixture to each PCR tube.
• Label and add each template to the corresponding PCR tube.
• Run PCR under appropriate Genotyping Program
• Load samples in gel and run on 130V (horizontally.)