TORC1 Kinase Buffer

mTORC1 Kinase Buffer (for 10mL lysis buffer)

	Final Concentration	per 10 mL	Stock	Location
HEPES, pH 7.4	25 mM	250 uL	1M	4C
KCl	50 mM	500 uL	1M	Shelf
MgCl2	10 mM	100 uL	1M	Shelf
ATP	250 uM	25 uL	100mM	-20

see PMID 19200882