TBST

10X TBS Buffer

To make 1X TBS, dilue 10X with water, and add 10 mL/10L Tween 20 (Sigma P7949-500ml)

Component	Weight per 10 L	Catalog Number
Tris Base	606 g	Sigma T6066-5KG
NaCl	877 g	Sigma S7653-5KG
HCl	360 mL	Sigma 258148-2.5L