KRBH Buffer

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Final Concentration per 500 mL Stock Location
HEPES pH 7.4 30mM 15mL 1M
Sodium bicarbonate 10mM 5mL 1M Make fresh from powder
Sodium chloride 120mM 15mL 4M
Potassium phosphate 4mM 2mL 1M
Magnesium sulfate 1mM 500uL 1M
Calcium chloride 1mM 500uL 1M
  • Bring up to 500 mL with ddH20
  • Sterile filter buffer and store at 4C