MTM1 Genotyping

See http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153

Primers

• MTM1 Fwd: GGC CCC ATT AAG GGA AGA GT
• MTM1 WT Rev: GAT CTT CTG GGA AAG GCA CA
• MTM1 Mut Rev: GCC AGA GGC CAC TTG TGT AG

PCR Program

• 94 for 2 min
• 10 cycles of:
  • 94 for 20sec
  • 65 for 15sec with -0.5 C per cycle
  • 68 for 10sec
• 28 cycles of:
  • 94 for 15sec
  • 60 for 15sec
  • 72 for 10sec
• 72 for 1 min
• 4 hold