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- 2xHNG Buffer
- 3T3-L1 Adipocyte Fractionation
- 3T3-L1 Plasma Membrane Isolation
- 4EBP1/2 Genotyping
- 50X TAE Buffer
- ACSF Media
- Acetate Incorporation into Lipid
- Acid Etching Coverslips
- Acrylamide Solution
- Actinomycin D
- Adding New Personnel to Animal Protocol
- Adipocyte Cell Counting with ImageJ
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
- Amitryptyline Treatment of Mice
- Amylose pull-down of glycogen-bound proteins
- Analysing Sequencing Results with SeqMan
- Analysis of FRET Images
- Antisense Oligo Knockdown in Mice
- Assessing isoproterenol-stimulated whole-body lipolysis in vivo
- BDNF PCR
- Bradford Assay
- CAP Genotyping
- CHAPS Lysis Buffer
- CPY Assay
- Cesium Chloride Preparation of DNA
- ChIP Elution Buffer
- Check Clones for Correct Mutation
- Chemical Hygiene Plan
- Collecting and Storing Mouse Serum
- Colocalization
- Colony PCR
- Connect to Network Drives
- Cre Genotyping
- Cre PCR
- Creating New R Packages
- Culturing MIN6 Cells
- Culturing RAW 264.7 Cells
- Cycloheximide
- DNA Preparation from Tail Clip
- Data Management
- Designing Clones Using Benchling
- Designing and Generating CRISPR-Cas Mutants
- Designing siRNA for Knockdown
- Determining Cortisol Levels in Human Hair
- Determining Percent Purity
- Dexamethasone Treatment of Mice
- Differentiation of MEFs
- Dilution Buffer
- Dilution Formula
- Electroporation of 3T3-L1 Adipocytes
- Extraction of DNA from TRIZOL preparations
- FDB Muscle Dissection from Mice
- Farnham Lysis Buffer
- Finding and Ordering BAC Clones
- First Strand cDNA Synthesis (AB Kit)
- Fly Food Protocol
- French Press
- Fugene Transfection of 293T/COS Cells
- GDF15 Genotyping
- General Statistics
- Generating DMSO Stocks for Cell Culture
- Generating and Displaying an Electostatic Surface
- Generation of Figures in Illustrator
- Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap)
- Glucose Incorporation into Lipid and Glycogen
- Glycogen Determination from Cells
- Glycogen Determination from Tissues
- Glycogen Phosphorylase Assay
- Glycogen Synthase Assay
- H&E Staining of Mouse Tissue
- HNTG Buffer
- HPLC - Plumbing Setup
- Harvesting RNA from Cells grown in monolayer
- High Fat Diet Treatment
- High Salt Immune Complex Wash Buffer
- How to Find a Journal Article
- How to design primer pairs for determining the presence of CrispR-cas induced mutations
- Immobilizing a Protein on a CM5 Chip for SPR
- Induction Conditions
- Inositol Labeling and Lipid Extraction
- Installing Software to a UNIX Server
- Isolation of Mouse Embryonic Fibroblasts (MEFs)
- KRBH Buffer
- Ketone Body Tolerance Test
- LiCl Immune Complex Wash Buffer
- Lipid Transfection
- Lipofectamine Mediated Knockdown
- Lipofectamine Plasmid Transfection
- Lipofectamine Transfection of C2C12 Cells
- Liposome Binding Assay
- Low Salt Immune Complex Wash Buffer
- Luciferase Assay
- MTM1 Genotyping
- Maintanence of 18C Fly Stocks
- Maintenance of 18C Fly Stocks
- Maintenance of Fly Stocks
- Mammary Gland Fat and SVF Separation
- Maternal Particulate Exposure Breeding
- Matrigel Basement Membrane Matrix
- Mc4r-Cone Genotyping
- Measurement of Glycolysis and Respiration Using Seahorse
- Measuring Autophagy in Tissue Culture Cells
- Measuring Cross-Sectional Area (CSA)
- Measuring Grip Strength
- Measuring Phagocytosis using pHrodo Bioparticles
- Milk Creamatocrit
- Monitoring Food Intake
- Nr3c1 Genotyping
- Ob/ob Genotyping
- Oil red o stain for in vitro adipogenesis
- Ordering Barcodes for Mice
- Ordering Mice
- Ordering Prescriptions-University of Michigan
- PCR Amplification of DNA (KOD Polymerase)
- PCR Amplification with Platinum Taq High Fidelity
- Parsing XSQ Files
- Performing Drosophila Crosses
- Phosphate Buffered Saline
- Phred Quality Scores
- Ppp1r3c Genotyping
- Preparation of Chloroquine Stocks
- Preparation of DIG Labelled Probes
- Preparation of Mouse Muscle for Cryosectioning
- Preparation of Orthovanadate Stocks
- Preparation of RNA Sample from Cell Culture
- Preparation of Tail Samples (for Genotyping)
- Preparing an Adenoviral shRNA Clone
- Preparing an Agarose Gel
- Preparing and Sending Tissues to Histology Core
- Primer Design for qPCR
- Protein-Lipid Overlay Assay
- Purification of miRNA and mRNA with TRIzol
- QPCR - Lin Lab
- QPCR Analysis on ThermoCloud
- Quantification of Vacuolar Colocalization
- Quantifying Relative Expression from qRT-PCR
- RNA-seq Pipeline for Known Transcripts
- Rapamycin Stock Solution
- Rapid Isolation of Genomic DNA from Yeast
- Rate of drop analysis
- Restriction Enzyme Based Cloning - Ordering Primers
- SDS-PAGE Running Buffer
- SDS-PAGE Separating Gel Buffer
- SDS-PAGE Stacking Gel Buffer
- SREBF1 Genotyping Protocol
- SRY Genotyping
- Scanning and Analyzing Western Blots Using LiCor Odyssey
- Seahorse XF Media
- Sending Samples for DNA Sequencing
- Set up qPCR Run on Roche LightCycler
- Single fiber dissociation of intact rodent muscles
- Submitting Plasmids for Sequencing
- Surveyor Assay for Genomic DNA Mutations
- TAP Purification of Yeast Extracts
- TBST
- TC10b Genotyping
- TE Buffer
- TOPO Cloning
- TORC1 Kinase Buffer
- TSC-floxed Genotyping
- TaqMan SNP Array
- Taqman
- Transfer Buffer
- Triglyceride Assay from Cells and Tissues
- Triglyceride Assay from Drosophila (German Method)
- Triglyceride Assay from Tissue Culture Cells
- Using Bioconductor To Analyse Beadarray Data
- Using Bioconductor To Analyse Beadarray Data (lumi)
- Using Bioconductor To Analyse Microarray Data
- Using GSEA for Gene Set Analyses of Transcriptional Data
- Using Git
- Using Github and Git for Code
- Using ImageJ to Quantify Bands
- Using Mr Bayes to For Phlyogenetic Analysis
- Using South To Migrate a Django Database
- Using the MBNI Cluster
- Using the echoMRI
- Using the pH Meter
- Vinexin Genotyping
- Weigh-Suckle-Weigh Milk Volume Measurement
- Wiki new protocols
- YPD Media and Agar
- Yeast Invertase Assay
- Yeast Sch9 Phosphorylation Assay
- Yeast Selective Media and Agar
- Yeast Transfection (Small Scale)