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Showing below up to 235 results in range #1 to #235.

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  1. Adipocyte Cell Counting with ImageJ‏‎ (2 revisions)
  2. Adipose Tissue Nuclear Isolation‏‎ (2 revisions)
  3. Amylose pull-down of glycogen-bound proteins‏‎ (2 revisions)
  4. Cloning CRISPR-Cas Plasmids‏‎ (2 revisions)
  5. Colocalization‏‎ (2 revisions)
  6. Colony PCR‏‎ (2 revisions)
  7. Data Management‏‎ (2 revisions)
  8. Designing Clones Using Benchling‏‎ (2 revisions)
  9. Determining Percent Purity‏‎ (2 revisions)
  10. Dilution Buffer‏‎ (2 revisions)
  11. Dilution Formula‏‎ (2 revisions)
  12. French Press‏‎ (2 revisions)
  13. Glycogen Phosphorylase Assay‏‎ (2 revisions)
  14. H&E Staining‏‎ (2 revisions)
  15. HNTG Buffer‏‎ (2 revisions)
  16. HPLC - Plumbing Setup‏‎ (2 revisions)
  17. HPLC - Running a Sample or Series‏‎ (2 revisions)
  18. Isolation of Mouse Embryonic Fibroblasts (MEFs)‏‎ (2 revisions)
  19. Isolation of Single Fibers for Seahorse Assays‏‎ (2 revisions)
  20. Lipofectamine Transfection of C2C12 Cells‏‎ (2 revisions)
  21. Maintenance of 18C Fly Stocks‏‎ (2 revisions)
  22. Making LB Agar Plates‏‎ (2 revisions)
  23. Mammary Gland Fat and SVF Separation‏‎ (2 revisions)
  24. Measuring Grip Strength‏‎ (2 revisions)
  25. NADH NBT Staining‏‎ (2 revisions)
  26. Ordering Barcodes for Mice‏‎ (2 revisions)
  27. Ordering Mice‏‎ (2 revisions)
  28. Parsing XSQ Files‏‎ (2 revisions)
  29. Preparation of Chloroquine Stocks‏‎ (2 revisions)
  30. Preparation of DIG Labelled Probes‏‎ (2 revisions)
  31. Preparation of Orthovanadate Stocks‏‎ (2 revisions)
  32. Preparation of RNA Sample from Cell Culture‏‎ (2 revisions)
  33. Primary Adipocyte Isolation‏‎ (2 revisions)
  34. Protein-Lipid Overlay Assay‏‎ (2 revisions)
  35. QPCR Analysis on ThermoCloud‏‎ (2 revisions)
  36. Quantification by Absorbance at 280nm‏‎ (2 revisions)
  37. Rapamycin Stock Solution‏‎ (2 revisions)
  38. SOP- Chloroform‏‎ (2 revisions)
  39. SOP - Corrosives‏‎ (2 revisions)
  40. Set up qPCR Run on Roche LightCycler‏‎ (2 revisions)
  41. Single fiber dissociation of intact rodent muscles‏‎ (2 revisions)
  42. TSC-floxed Genotyping‏‎ (2 revisions)
  43. Transfer Buffer‏‎ (2 revisions)
  44. Triglyceride Assay from Drosophila (German Method)‏‎ (2 revisions)
  45. Using the MBNI Cluster‏‎ (2 revisions)
  46. Using the echoMRI‏‎ (2 revisions)
  47. Wiki new protocols‏‎ (2 revisions)
  48. Yeast Selective Media and Agar‏‎ (2 revisions)
  49. Yeast Transfection (Small Scale)‏‎ (2 revisions)
  50. Acrylamide Solution‏‎ (3 revisions)
  51. Affinity Purification of Proteins - FPLC‏‎ (3 revisions)
  52. Amitryptyline Treatment of Mice‏‎ (3 revisions)
  53. Back-Crossing of Mouse Strains‏‎ (3 revisions)
  54. CHAPS Lysis Buffer‏‎ (3 revisions)
  55. Cesium Chloride Preparation of DNA‏‎ (3 revisions)
  56. ChIP Elution Buffer‏‎ (3 revisions)
  57. Connect to Network Drives‏‎ (3 revisions)
  58. Deparaffinizing Histology Slides‏‎ (3 revisions)
  59. Determining Cortisol Levels in Human Hair‏‎ (3 revisions)
  60. Fly Food Protocol‏‎ (3 revisions)
  61. Freezing and Thawing S2 Cells‏‎ (3 revisions)
  62. GST-EEA1 Pulldown from Yeast‏‎ (3 revisions)
  63. GST Pulldown Assay‏‎ (3 revisions)
  64. Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap)‏‎ (3 revisions)
  65. Glycogen Determination from Cells‏‎ (3 revisions)
  66. HPLC - Designing a Method for a Sample or Series‏‎ (3 revisions)
  67. Installing Software to a UNIX Server‏‎ (3 revisions)
  68. Low Salt Immune Complex Wash Buffer‏‎ (3 revisions)
  69. MTM1 Genotyping‏‎ (3 revisions)
  70. Maintenance of Fly Stocks‏‎ (3 revisions)
  71. Matrigel Basement Membrane Matrix‏‎ (3 revisions)
  72. Mc4r-Cone Genotyping‏‎ (3 revisions)
  73. PAS Staining‏‎ (3 revisions)
  74. PCR Amplification of DNA (KOD Polymerase)‏‎ (3 revisions)
  75. Performing Drosophila Crosses‏‎ (3 revisions)
  76. Phosphate Buffered Saline‏‎ (3 revisions)
  77. Preparation of PEI Stocks‏‎ (3 revisions)
  78. Preparation of Protein Lysates from Cells‏‎ (3 revisions)
  79. Preparing an Agarose Gel‏‎ (3 revisions)
  80. Preparing and Sending Tissues to Histology Core‏‎ (3 revisions)
  81. Purification of GST Fusion Proteins by GSTrap‏‎ (3 revisions)
  82. Rapamycin Sensitivity Assay of Yeast‏‎ (3 revisions)
  83. Restriction Enzyme Based Cloning‏‎ (3 revisions)
  84. SOP - BVT.2733‏‎ (3 revisions)
  85. Scanning and Analyzing Western Blots Using LiCor Odyssey‏‎ (3 revisions)
  86. Surveyor Assay for Genomic DNA Mutations‏‎ (3 revisions)
  87. Thawing Culture Cells‏‎ (3 revisions)
  88. Triglyceride Assay from Tissue Culture Cells‏‎ (3 revisions)
  89. Using Github and Git for Code‏‎ (3 revisions)
  90. Verifying Kan Deletion Mutants from Genomic DNA‏‎ (3 revisions)
  91. Vinexin Genotyping‏‎ (3 revisions)
  92. YPD Media and Agar‏‎ (3 revisions)
  93. 5' RACE‏‎ (4 revisions)
  94. 50X TAE Buffer‏‎ (4 revisions)
  95. ACSF Media‏‎ (4 revisions)
  96. Buffer/CHAPS Lysis Buffer‏‎ (4 revisions - redirect page)
  97. CPY Assay‏‎ (4 revisions)
  98. Cre PCR‏‎ (4 revisions)
  99. Culturing RAW 264.7 Cells‏‎ (4 revisions)
  100. Culturing and Differentiating C2C12 Cells‏‎ (4 revisions)
  101. Designing siRNA for Knockdown‏‎ (4 revisions)
  102. Electroporation of 3T3-L1 Adipocytes‏‎ (4 revisions)
  103. Extraction of DNA from TRIZOL preparations‏‎ (4 revisions)
  104. FDB Muscle Dissection from Mice‏‎ (4 revisions)
  105. Generating and Amplifying Adenoviral Constructs‏‎ (4 revisions)
  106. Glycogen Synthase Assay‏‎ (4 revisions)
  107. GoTaq PCR Genotyping‏‎ (4 revisions)
  108. Harvesting RNA from Cells grown in monolayer‏‎ (4 revisions)
  109. Inositol Labeling and Lipid Extraction‏‎ (4 revisions)
  110. MTORC1 Kinase Assay‏‎ (4 revisions)
  111. Measuring Cross-Sectional Area (CSA)‏‎ (4 revisions)
  112. Measuring Fasting Insulin‏‎ (4 revisions)
  113. Osmotic Minipump Implantation‏‎ (4 revisions)
  114. Production of LentiCRISPR Viruses‏‎ (4 revisions)
  115. Seahorse XF Media‏‎ (4 revisions)
  116. Sequence Alignment for Phylogenetic Analysis‏‎ (4 revisions)
  117. Serum Total Cholesterol Assay‏‎ (4 revisions)
  118. Transformation of Bacteria‏‎ (4 revisions)
  119. Using Mr Bayes to For Phlyogenetic Analysis‏‎ (4 revisions)
  120. Weigh-Suckle-Weigh Milk Volume Measurement‏‎ (4 revisions)
  121. Writing a Paper and Uploading Code to Github Repository‏‎ (4 revisions)
  122. 3T3-L1 Plasma Membrane Isolation‏‎ (5 revisions)
  123. Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)‏‎ (5 revisions)
  124. Buffer/RIPA‏‎ (5 revisions - redirect page)
  125. Chromatin Immunoprecipitation for Tissue Samples‏‎ (5 revisions)
  126. DNA Preparation from Tail Clip‏‎ (5 revisions)
  127. First Strand cDNA Synthesis (AB Kit)‏‎ (5 revisions)
  128. Inositol Labelling of Yeast Cells‏‎ (5 revisions)
  129. Ketone Body Tolerance Test‏‎ (5 revisions)
  130. Luciferase Assay‏‎ (5 revisions)
  131. Milk Creamatocrit‏‎ (5 revisions)
  132. Mutagenesis‏‎ (5 revisions)
  133. Preparing a SDS-PAGE Gel‏‎ (5 revisions)
  134. Primer Design for qPCR‏‎ (5 revisions)
  135. Pyruvate Tolerance Test‏‎ (5 revisions)
  136. RT-PCR primer design for ChIP‏‎ (5 revisions)
  137. SHROB Genotyping‏‎ (5 revisions)
  138. Seahorse - Mitochondrial Stress Test‏‎ (5 revisions)
  139. Statistical Analysis‏‎ (5 revisions)
  140. Submitting Plasmids for Sequencing‏‎ (5 revisions)
  141. Yeast Transformation‏‎ (5 revisions)
  142. 4EBP1/2 Genotyping‏‎ (6 revisions)
  143. Dexamethasone Treatment of Mice‏‎ (6 revisions)
  144. Fugene Transfection of 293T/COS Cells‏‎ (6 revisions)
  145. Generating DMSO Stocks for Cell Culture‏‎ (6 revisions)
  146. Generation of Figures in Illustrator‏‎ (6 revisions)
  147. Lipofectamine Plasmid Transfection‏‎ (6 revisions)
  148. Ob/ob Genotyping‏‎ (6 revisions)
  149. Perfusion of Mouse‏‎ (6 revisions)
  150. Purification of GST-HA-S6K‏‎ (6 revisions)
  151. QPCR - Lin Lab‏‎ (6 revisions)
  152. Quantification of miRNA by SYBR Green qPCR‏‎ (6 revisions)
  153. RIPA Buffer‏‎ (6 revisions)
  154. Rate of drop analysis‏‎ (6 revisions)
  155. Restriction Enzyme Based Cloning - Ordering Primers‏‎ (6 revisions)
  156. SOP - Ethidium Bromide‏‎ (6 revisions)
  157. SOP - Rapamycin‏‎ (6 revisions)
  158. Set up qPCR Run on Thermo Cloud and QuantStudio‏‎ (6 revisions)
  159. Yeast Sch9 Phosphorylation Assay‏‎ (6 revisions)
  160. 3T3-L1 Adipocyte Fractionation‏‎ (7 revisions)
  161. Acetate Incorporation into Lipid‏‎ (7 revisions)
  162. Differentiation of MEFs‏‎ (7 revisions)
  163. FM4-64 Labeling of Yeast Cells‏‎ (7 revisions)
  164. Finding and Ordering BAC Clones‏‎ (7 revisions)
  165. H&E Staining of Mouse Tissue‏‎ (7 revisions)
  166. Induction Conditions‏‎ (7 revisions)
  167. Lipofectamine Mediated Knockdown‏‎ (7 revisions)
  168. Nr3c1 Genotyping‏‎ (7 revisions)
  169. Oil red o stain for in vitro adipogenesis‏‎ (7 revisions)
  170. Preparing an Adenoviral shRNA Clone‏‎ (7 revisions)
  171. SOP - Bunsen Burners‏‎ (7 revisions)
  172. SOP - Cytotoxins and Antineoplastic Drugs‏‎ (7 revisions)
  173. SOP - Sensitizers‏‎ (7 revisions)
  174. SREBF1 Genotyping Protocol‏‎ (7 revisions)
  175. Using Bioconductor To Analyse Beadarray Data (lumi)‏‎ (7 revisions)
  176. Culturing S2 Cells‏‎ (8 revisions)
  177. Generating and Displaying an Electostatic Surface‏‎ (8 revisions)
  178. High Fat Diet Treatment‏‎ (8 revisions)
  179. Purification of GST Fusion Proteins‏‎ (8 revisions)
  180. SOP - Biosafety Cabinets‏‎ (8 revisions)
  181. SOP - Cryogenic Materials‏‎ (8 revisions)
  182. SOP - Formalin and Paraformaldehyde‏‎ (8 revisions)
  183. SOP - Needle Capping‏‎ (8 revisions)
  184. SOP - Vacuum Pumps‏‎ (8 revisions)
  185. Designing and Preparing dsRNA‏‎ (9 revisions)
  186. DsRNA Mediated Knockdown of S2 Cells‏‎ (9 revisions)
  187. Glucose Uptake Assay‏‎ (9 revisions)
  188. Immunofluoresence‏‎ (9 revisions)
  189. Immunoprecipitation‏‎ (9 revisions)
  190. RNA-seq Pipeline for Known Transcripts‏‎ (9 revisions)
  191. Rapamycin Injections‏‎ (9 revisions)
  192. SOP - Carcinogens‏‎ (9 revisions)
  193. SOP - Flammables and Combustibles‏‎ (9 revisions)
  194. SOP - Tamoxifen‏‎ (9 revisions)
  195. Spill Response Procedures for Infectious agents and Recombinant DNA‏‎ (9 revisions)
  196. Tamoxifen Injections for Inducible Knockouts‏‎ (9 revisions)
  197. Glucose Incorporation into Lipid and Glycogen‏‎ (10 revisions)
  198. PCR Amplification of DNA‏‎ (10 revisions)
  199. Paraffin Embedding of Tissue Samples‏‎ (10 revisions)
  200. Preparation of Protein Lysates from Mouse Tissues‏‎ (10 revisions)
  201. SOP - Centrifuge Safety‏‎ (10 revisions)
  202. SOP - Compressed Gases‏‎ (10 revisions)
  203. SOP - Irritants‏‎ (10 revisions)
  204. Surface Plasmon Resonance - Protein Lipid Interactions‏‎ (10 revisions)
  205. Bradford Assay‏‎ (11 revisions)
  206. Glucose Tolerance Test‏‎ (11 revisions)
  207. Insulin Tolerance Test‏‎ (11 revisions)
  208. SOP - Chloroform‏‎ (11 revisions)
  209. SOP - Phenol‏‎ (11 revisions)
  210. Analysing Data on Armis or Great Lakes using Rmd Files‏‎ (12 revisions)
  211. Preparation of Tail Samples (for Genotyping)‏‎ (12 revisions)
  212. SOP - Electrophoresis‏‎ (12 revisions)
  213. Designing and Generating CRISPR-Cas Mutants‏‎ (13 revisions)
  214. QPCR‏‎ (14 revisions)
  215. Glycogen Determination from Tissues‏‎ (15 revisions)
  216. Preparation of RNA Samples from Mouse Tissues‏‎ (15 revisions)
  217. Preparing Cell Lysates‏‎ (15 revisions)
  218. SOP - B-mercaptoethanol‏‎ (15 revisions)
  219. Splitting Cells‏‎ (15 revisions)
  220. SOP - Animal Anesthetics‏‎ (16 revisions)
  221. SOP - Autoclave‏‎ (16 revisions)
  222. Differentiation of 3T3-L1 Cells‏‎ (18 revisions)
  223. SOP - Bleach‏‎ (18 revisions)
  224. Assessing isoproterenol-stimulated whole-body lipolysis in vivo‏‎ (19 revisions)
  225. Real Time PCR From Cell Culture‏‎ (20 revisions)
  226. Triglyceride Assay from Cells and Tissues‏‎ (20 revisions)
  227. Genotyping Program‏‎ (21 revisions)
  228. PCR Analysis of Tail DNA‏‎ (21 revisions)
  229. Western Blotting‏‎ (21 revisions)
  230. Using Bioconductor To Analyse Beadarray Data‏‎ (22 revisions)
  231. Safety and Animal Training‏‎ (23 revisions)
  232. Using Bioconductor To Analyse Microarray Data‏‎ (28 revisions)
  233. TaqMan SNP Assay‏‎ (33 revisions)
  234. Chromatin Immunoprecipitation‏‎ (79 revisions)
  235. Main Page‏‎ (80 revisions)

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