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See [[Special:Categories]] for as listing of protocols by category. | See [[Special:Categories]] for as listing of protocols by category. | ||
==Cell and Tissue Culture== | ==Lab Safety== | ||
* For notes about required training courses see [[ Safety and Animal Training ]] | |||
* For lab-specific standard operating procedures see [[ :Category:SOP]] | |||
==Common Protocols== | |||
===Cell and Tissue Culture=== | |||
*[[Splitting Cells]] | *[[Splitting Cells]] | ||
*[[Generating DMSO Stocks for Cell Culture]] | *[[Generating DMSO Stocks for Cell Culture]] | ||
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*[[Inositol Labeling and Lipid Extraction]] | *[[Inositol Labeling and Lipid Extraction]] | ||
==Cloning and Molecular Biology== | ===Cloning and Molecular Biology=== | ||
*[[Transformation of Bacteria]] | *[[Transformation of Bacteria]] | ||
*[[Mutagenesis]] | *[[Mutagenesis]] | ||
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*[[Designing and Generating CRISPR-Cas Mutants]] | *[[Designing and Generating CRISPR-Cas Mutants]] | ||
==Cell Biology== | ===Cell Biology=== | ||
*[[Immunofluoresence]] | *[[Immunofluoresence]] | ||
**[[Colocalization]] | **[[Colocalization]] | ||
*[[FM4-64 Labeling of Yeast Cells]] | *[[FM4-64 Labeling of Yeast Cells]] | ||
==Protein Analysis== | ===Protein Analysis=== | ||
*[[Preparing a SDS-PAGE Gel]] | *[[Preparing a SDS-PAGE Gel]] | ||
*[[Western Blotting]] | *[[Western Blotting]] | ||
*[[Surface Plasmon Resonance - Protein Lipid Interactions]] | *[[Surface Plasmon Resonance - Protein Lipid Interactions]] | ||
===Protein Quantification=== | ====Protein Quantification==== | ||
*[[Bradford Assay]] | *[[Bradford Assay]] | ||
*[[Quantification by Absorbance at 280nm]] | *[[Quantification by Absorbance at 280nm]] | ||
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*[[Using ImageJ to Quantify Bands]] | *[[Using ImageJ to Quantify Bands]] | ||
===Protein Purification=== | ====Protein Purification==== | ||
*[[French Press]] | *[[French Press]] | ||
*[[Purification of GST Fusion Proteins]] | *[[Purification of GST Fusion Proteins]] | ||
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*[[Triglyceride Assay from Drosophila (German Method)]] | *[[Triglyceride Assay from Drosophila (German Method)]] | ||
==Transcriptional Analysis== | ===Transcriptional Analysis=== | ||
*[[Real Time PCR From Cell Culture]] | *[[Real Time PCR From Cell Culture]] | ||
*[[Harvesting RNA from Cells grown in monolayer]] | *[[Harvesting RNA from Cells grown in monolayer]] | ||
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*[[Using Bioconductor To Analyse Microarray Data]] | *[[Using Bioconductor To Analyse Microarray Data]] | ||
==Mouse/Fly Protocols== | ===Mouse/Fly Protocols=== | ||
*[[Maternal Particulate Exposure Breeding]] | *[[Maternal Particulate Exposure Breeding]] | ||
==Fly Stocks== | ===Fly Stocks=== | ||
*[[Maintenance of 18C Fly Stocks]] | *[[Maintenance of 18C Fly Stocks]] | ||
*[[Fly Food Protocol]] | *[[Fly Food Protocol]] | ||
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*[[Monitoring Food Intake]] | *[[Monitoring Food Intake]] | ||
==Tissue Preparation== | ===Tissue Preparation=== | ||
*[[Harvesting Mouse Tissue]] | *[[Harvesting Mouse Tissue]] | ||
*[[Preparation of Protein Lysates from Mouse Tissues]] | *[[Preparation of Protein Lysates from Mouse Tissues]] | ||
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*[[Perfusion of Mouse]] | *[[Perfusion of Mouse]] | ||
==Media and Buffers== | ===Media and Buffers=== | ||
===Yeast Media=== | ====Yeast Media==== | ||
*[[YPD Media and Agar]] | *[[YPD Media and Agar]] | ||
*[[Yeast Selective Media and Agar]] | *[[Yeast Selective Media and Agar]] | ||
===Buffers=== | ====Buffers==== | ||
====Lysis Buffers==== | =====Lysis Buffers===== | ||
*[[KRBH Buffer]] | *[[KRBH Buffer]] | ||
*[[RIPA Buffer]] | *[[RIPA Buffer]] | ||
*[[CHAPS Lysis Buffer]] | *[[CHAPS Lysis Buffer]] | ||
====SDS-PAGE Buffers==== | =====SDS-PAGE Buffers===== | ||
*[[SDS-PAGE Separating Gel Buffer]] | *[[SDS-PAGE Separating Gel Buffer]] | ||
*[[SDS-PAGE Stacking Gel Buffer]] | *[[SDS-PAGE Stacking Gel Buffer]] | ||
*[[TORC1 Kinase Buffer]] | *[[TORC1 Kinase Buffer]] | ||
*[[Acrylamide Solution]] | *[[Acrylamide Solution]] | ||
====Other Buffers==== | =====Other Buffers===== | ||
*[[KRBH Buffer]] | *[[KRBH Buffer]] | ||
*[[MTORC1 Kinase Assay]] | *[[MTORC1 Kinase Assay]] | ||