User contributions
9 January 2018
High Salt Immune Complex Wash Buffer
Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | SDS || 0.1% || 150uL || 10% || Solutions Shelf |- | Triton X-100 || 1% || 1.5mL..."
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
Low Salt Immune Complex Wash Buffer
no edit summary
+6
LiCl Immune Complex Wash Buffer
Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | NP40 || 1% || 1.5mL || 1..."
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
Low Salt Immune Complex Wash Buffer
no edit summary
-21
Low Salt Immune Complex Wash Buffer
Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | Example || Example || Ex..."
Chromatin Immunoprecipitation
Prior to starting this section:
-11
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+3
Chromatin Immunoprecipitation
Reverse Crosslinks of Protein/DNA Complexes to Free DNA
+6
Chromatin Immunoprecipitation
Prior to starting this section:
-1
Chromatin Immunoprecipitation
Immunoprecipitation
+16
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+6
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+19
Chromatin Immunoprecipitation
Immunoprecipitation
+58
Chromatin Immunoprecipitation
no edit summary
-4
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
-32
Chromatin Immunoprecipitation
Immunoprecipitation
+3,234
5 January 2018
15 December 2017
Preparation of Protein Lysates from Cells
no edit summary
-161
Preparation of Protein Lysates from Cells
Created page with "==Materials== *RIPA Buffer (see RIPA) or other Lysis buffer. Add protease inhibitors. *Cells (fresh or frozen) ==Protocol== #Cool centrifuge to 4C #If cells..."
24 October 2017
Submitting Plasmids for Sequencing
no edit summary
Submitting Plasmids for Sequencing
no edit summary
+159
19 October 2017
Cloning CRISPR-Cas Plasmids
no edit summary
+114
Submitting Plasmids for Sequencing
no edit summary
+32
Submitting Plasmids for Sequencing
no edit summary
+752
Submitting Plasmids for Sequencing
Created page with "*Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul *These should be submitted in 1.5mL tubes *Go to https://client-seqcore.brcf.med.um..."
11 August 2017
8 August 2017
4 August 2017
29 June 2017
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+5
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+116
12 June 2017
28 April 2017
Tamoxifen Injections for Inducible Knockouts
no edit summary
+1
Tamoxifen Injections for Inducible Knockouts
no edit summary
+85
27 April 2017
Tamoxifen Injections for Inducible Knockouts
no edit summary
+29
Tamoxifen Injections for Inducible Knockouts
no edit summary
+2
Tamoxifen Injections for Inducible Knockouts
no edit summary
+4
Tamoxifen Injections for Inducible Knockouts
no edit summary
+219
Tamoxifen Injections for Inducible Knockouts
no edit summary
+1,325
2 February 2017
Assessing isoproterenol-stimulated whole-body lipolysis in vivo
Reagents Needed
+3
Assessing isoproterenol-stimulated whole-body lipolysis in vivo
Experimental protocol
+90
31 January 2017
10 November 2016
Spill Response Procedures for Infectious agents and Recombinant DNA
Certification
+85
SOP - Vacuum Pumps
Training of Personnel
+85
SOP - Sensitizers
Certification
+85
SOP - Irritants
Certification
+86
SOP - Compressed Gases
Certification
+85