Changes

* Inject 40 50 mg/kg on three five consecutive days, at the same timeeach day. The time is important, otherwise they will overdose if you inject too close together. * To determine how much to inject weigh the mouse and inject 4 5 uL/g of mouse. This means a 25 g mouse will get an injection of 100 125 uL.* Give yourself plenty of time to fill needles because the corn oil takes a long time to aspirate--watch for bubbles.* Tamoxifen can be stored at room temperature for up to a week. * Notes about transferring mice: ** Mice must be transferred to the biocontainment room during injections and for an additional 72 hrs post final injection. ** The husbandry staff need to know a few days prior to transfer so they can have the room ready for occupancy (i.e. everything stocked and agent filled), for this email sanderjo@med.umich.edu to tell him '''when''' and '''how many cages''' you you plan to transfer. ** If you are introducing a new chemical or biological hazard, they need to be made aware of this. ** There are special barcodes for this room so you must change them out when you transfer in and then again change them to the regular ones when transferring back out. ** Remember to mark the cage with the appropriate sticker (biological or chemical hazard) and place appropriate acetate with required information (name of agent, dates of administration, and dose)---if you are treating the water with a drug the bottle must be marked was well.** Make sure to provide animals with food and water.** Additional PPE is required and highly monitored in this area, make sure to abide with these regulations!