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Tamoxifen Injections for Inducible Knockouts

219 bytes added, 15:45, 27 April 2017
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* Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil)-- must wear nitrile gloves when dealing with tamoxifen.
* Inject 50 mg/kg on five consecutive days, at the same time each day. The time is important, otherwise they will overdose if you inject too close together.
* To determine how much to inject weigh the mouse and inject 5 uL/g of mouse. This means a 25 g mouse will get an injection of 125 uL.
* Give yourself plenty of time to fill needles because the corn oil takes a long time to aspirate--watch for bubbles.
* Tamoxifen can be stored at room temperature for up to a week.
* The initial weights can be used for the rest of the week when determining injection volume, unless there is reason to believe they will be changing drastically.
* Notes about transferring mice:
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