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High Salt Immune Complex Wash Buffer

402 bytes added, 20:05, 9 January 2018
Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | SDS || 0.1% || 150uL || 10% || Solutions Shelf |- | Triton X-100 || 1% || 1.5mL..."
{| class="wikitable"
|-
! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location
|-
| SDS || 0.1% || 150uL || 10% || Solutions Shelf
|-
| Triton X-100 || 1% || 1.5mL || 10% || Solutions Shelf (borrowed from Seo Lab to make stock)
|-
| EDTA || 2mM || 60uL || 500mM || Solutions Shelf
|-
| Tris || 20mM || 300uL || 1M || Solutions Shelf
|-
| NaCl || 500mM || 1.875mL || 4M || Solutions Shelf
|}
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