User contributions for Iharvey

This user is currently blocked. The latest block log entry is provided below for reference:

18:39, 26 January 2023 Davebrid talk contribs blocked Iharvey talk contribs with an expiration time of indefinite (account creation disabled)

15:2315:23, 10 May 2018 diff hist +48 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads current
15:2115:21, 10 May 2018 diff hist +6 Chromatin Immunoprecipitation →Prior to starting this section:
15:2015:20, 10 May 2018 diff hist +18 Chromatin Immunoprecipitation →Reverse Crosslinks of Protein/DNA Complexes to Free DNA

22:1322:13, 9 January 2018 diff hist −8 Chromatin Immunoprecipitation →Prior to starting this section:
22:0922:09, 9 January 2018 diff hist 0 ChIP Elution Buffer No edit summary current
22:0922:09, 9 January 2018 diff hist +313 ChIP Elution Buffer No edit summary
21:4121:41, 9 January 2018 diff hist +6 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
21:4121:41, 9 January 2018 diff hist +2 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
21:3221:32, 9 January 2018 diff hist +57 Chromatin Immunoprecipitation →Before You Start
21:2921:29, 9 January 2018 diff hist +337 Dilution Buffer No edit summary current
20:1720:17, 9 January 2018 diff hist +71 N ChIP Elution Buffer Created page with "10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water."
20:1520:15, 9 January 2018 diff hist +79 N Dilution Buffer Created page with "0.01% SDS, 1.1% Triton X- 100, 1.2mM EDTA, 16.7mM Tris-HCl, pH 8.1, 167mM NaCl."
20:1520:15, 9 January 2018 diff hist +22 Chromatin Immunoprecipitation →Buffers and Solutions Needed
20:1220:12, 9 January 2018 diff hist +139 Chromatin Immunoprecipitation →Buffers and Solutions Needed
20:1120:11, 9 January 2018 diff hist −79 Chromatin Immunoprecipitation →Buffers and Solutions Needed
20:0920:09, 9 January 2018 diff hist +196 N TE Buffer Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | Tris|| 10mM|| 150uL || 1M || Solutions Shelf |- | EDTA || 1mM || 30uL || 500mM..." current
20:0720:07, 9 January 2018 diff hist +4 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
20:0520:05, 9 January 2018 diff hist +402 N High Salt Immune Complex Wash Buffer Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | SDS || 0.1% || 150uL || 10% || Solutions Shelf |- | Triton X-100 || 1% || 1.5mL..." current
19:5919:59, 9 January 2018 diff hist +4 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
19:5819:58, 9 January 2018 diff hist +6 Low Salt Immune Complex Wash Buffer No edit summary current
19:5419:54, 9 January 2018 diff hist +355 N LiCl Immune Complex Wash Buffer Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | NP40 || 1% || 1.5mL || 1..." current
19:5419:54, 9 January 2018 diff hist +4 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
19:5319:53, 9 January 2018 diff hist +4 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
19:5219:52, 9 January 2018 diff hist −21 Low Salt Immune Complex Wash Buffer No edit summary
19:4819:48, 9 January 2018 diff hist +376 N Low Salt Immune Complex Wash Buffer Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | Example || Example || Ex..."
19:3019:30, 9 January 2018 diff hist −11 Chromatin Immunoprecipitation →Prior to starting this section:
19:3019:30, 9 January 2018 diff hist +3 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
19:2819:28, 9 January 2018 diff hist +6 Chromatin Immunoprecipitation →Reverse Crosslinks of Protein/DNA Complexes to Free DNA
19:2819:28, 9 January 2018 diff hist −1 Chromatin Immunoprecipitation →Prior to starting this section:
19:2719:27, 9 January 2018 diff hist +16 Chromatin Immunoprecipitation →Immunoprecipitation
19:2419:24, 9 January 2018 diff hist +6 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
19:2319:23, 9 January 2018 diff hist +19 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
19:1919:19, 9 January 2018 diff hist +58 Chromatin Immunoprecipitation →Immunoprecipitation
19:1719:17, 9 January 2018 diff hist −4 Chromatin Immunoprecipitation No edit summary
19:1419:14, 9 January 2018 diff hist −32 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
19:1119:11, 9 January 2018 diff hist +3,234 Chromatin Immunoprecipitation →Immunoprecipitation

21:4321:43, 5 January 2018 diff hist +2 Preparation of Protein Lysates from Cells No edit summary current

18:3318:33, 15 December 2017 diff hist −161 Preparation of Protein Lysates from Cells No edit summary
18:2818:28, 15 December 2017 diff hist +1,041 N Preparation of Protein Lysates from Cells Created page with "==Materials== *RIPA Buffer (see RIPA) or other Lysis buffer. Add protease inhibitors. *Cells (fresh or frozen) ==Protocol== #Cool centrifuge to 4C #If cells..."