162
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Changes
→Immunoprecipitation
14. Snap-freeze the sample in liquid nitrogen and store at -80°C, or do not freeze and continue with the immunoprecipitation steps below.
===Immunoprecipitation===(taken from Millipore EZ ChIP protocol)
''Perform all steps in an ice bucket or in the cold room at 4°C.''
* LiCl Immune Complex Wash Buffer (Catalog # 20-156), one wash
* TE Buffer (Catalog # 20-157), two washes
• Bring 1 M NaHCO3 to room temperature. A precipitate may be observed but will go into solution once room temperature is achieved. The 1 M NaHCO3 can be vortexed.
• Set water bath to 65°C for use in Section E.
