Changes

Jump to: navigation, search

Chromatin Immunoprecipitation

4 bytes removed, 19:17, 9 January 2018
no edit summary
supernatant fraction.
# Wash the Protein G Agarose-antibody/chromatin complex by resuspending the beads in 1 mL each of the cold buffers in the order listed below and incubating for 3-5 minutes on a rotating platform followed by brief centrifugation (3000-5000 x g for 1 minute) and careful removal of the supernatant fraction:
a. * Low Salt Immune Complex Wash Buffer (Catalog # 20-154), one washb. * High Salt Immune Complex Wash Buffer (Catalog # 20-155), one washc. * LiCl Immune Complex Wash Buffer (Catalog # 20-156), one washd. * TE Buffer (Catalog # 20-157), two washes
D. Elution of Protein/DNA Complexes
Prior to starting this section:
Iharvey
162
edits
Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/Special:MobileDiff/1412"

Navigation menu