162
edits
Changes
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
28. Wash the Protein G Agarose-antibody/chromatin complex by resuspending the beads in 1 mL each of the cold buffers in the order listed below and incubating for 3-5 minutes on a rotating platform followed by brief centrifugation (3000-5000 x g for 1 minute) and careful removal of the supernatant fraction:
** [[Low Salt Immune Complex Wash Buffer]] (Catalog # 20-154), one wash
** [[High Salt Immune Complex Wash Buffer ]] (Catalog # 20-155), one wash
** [[LiCl Immune Complex Wash Buffer]] (Catalog # 20-156), 3-5 washes
** TE Buffer (Catalog # 20-157), two washes