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  1. 2xHNG Buffer
  2. 3T3-L1 Adipocyte Fractionation
  3. 3T3-L1 Plasma Membrane Isolation
  4. 4EBP1/2 Genotyping
  5. 50X TAE Buffer
  6. ACSF Media
  7. Acetate Incorporation into Lipid
  8. Acid Etching Coverslips
  9. Acrylamide Solution
  10. Actinomycin D
  11. Adding New Personnel to Animal Protocol
  12. Adipocyte Cell Counting with ImageJ
  13. Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
  14. Amitryptyline Treatment of Mice
  15. Amylose pull-down of glycogen-bound proteins
  16. Analysing Sequencing Results with SeqMan
  17. Analysis of FRET Images
  18. Antisense Oligo Knockdown in Mice
  19. Assessing isoproterenol-stimulated whole-body lipolysis in vivo
  20. BDNF PCR
  21. Bradford Assay
  22. CAP Genotyping
  23. CHAPS Lysis Buffer
  24. CPY Assay
  25. Cesium Chloride Preparation of DNA
  26. ChIP Elution Buffer
  27. Check Clones for Correct Mutation
  28. Chemical Hygiene Plan
  29. Collecting and Storing Mouse Serum
  30. Colocalization
  31. Colony PCR
  32. Connect to Network Drives
  33. Cre Genotyping
  34. Cre PCR
  35. Creating New R Packages
  36. Culturing MIN6 Cells
  37. Culturing RAW 264.7 Cells
  38. Cycloheximide
  39. DNA Preparation from Tail Clip
  40. Data Management
  41. Designing Clones Using Benchling
  42. Designing and Generating CRISPR-Cas Mutants
  43. Designing siRNA for Knockdown
  44. Determining Cortisol Levels in Human Hair
  45. Determining Percent Purity
  46. Dexamethasone Treatment of Mice
  47. Differentiation of MEFs
  48. Dilution Buffer
  49. Dilution Formula
  50. Electroporation of 3T3-L1 Adipocytes
  51. Extraction of DNA from TRIZOL preparations
  52. FDB Muscle Dissection from Mice
  53. Farnham Lysis Buffer
  54. Finding and Ordering BAC Clones
  55. First Strand cDNA Synthesis (AB Kit)
  56. Fly Food Protocol
  57. French Press
  58. Fugene Transfection of 293T/COS Cells
  59. GDF15 Genotyping
  60. General Statistics
  61. Generating DMSO Stocks for Cell Culture
  62. Generating and Displaying an Electostatic Surface
  63. Generation of Figures in Illustrator
  64. Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap)
  65. Glucose Incorporation into Lipid and Glycogen
  66. Glycogen Determination from Cells
  67. Glycogen Determination from Tissues
  68. Glycogen Phosphorylase Assay
  69. Glycogen Synthase Assay
  70. H&E Staining of Mouse Tissue
  71. HNTG Buffer
  72. HPLC - Plumbing Setup
  73. Harvesting RNA from Cells grown in monolayer
  74. High Fat Diet Treatment
  75. High Salt Immune Complex Wash Buffer
  76. How to Find a Journal Article
  77. How to design primer pairs for determining the presence of CrispR-cas induced mutations
  78. Immobilizing a Protein on a CM5 Chip for SPR
  79. Induction Conditions
  80. Inositol Labeling and Lipid Extraction
  81. Installing Software to a UNIX Server
  82. Isolation of Mouse Embryonic Fibroblasts (MEFs)
  83. KRBH Buffer
  84. Ketone Body Tolerance Test
  85. LiCl Immune Complex Wash Buffer
  86. Lipid Transfection
  87. Lipofectamine Mediated Knockdown
  88. Lipofectamine Plasmid Transfection
  89. Lipofectamine Transfection of C2C12 Cells
  90. Liposome Binding Assay
  91. Low Salt Immune Complex Wash Buffer
  92. Luciferase Assay
  93. MTM1 Genotyping
  94. Maintanence of 18C Fly Stocks
  95. Maintenance of 18C Fly Stocks
  96. Maintenance of Fly Stocks
  97. Mammary Gland Fat and SVF Separation
  98. Maternal Particulate Exposure Breeding
  99. Matrigel Basement Membrane Matrix
  100. Mc4r-Cone Genotyping

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