User contributions
4 May 2009
Help:Contents
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+20
Help:Contents
Editing
+12
Help:Contents
no edit summary
+42
Help:Preventing access
Created page with '{{languages}} For help customizing user rights, see Manual:User rights. This page contains examples useful for restricting access. Most of the examples need changes to [[Ma...'
Bradford Assay
Reference
+2
Bradford Assay
no edit summary
+69
Help:Contents
no edit summary
+26
Help:Contents
Editing
+21
Help:Wikitext examples
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-11
Help:Contents
no edit summary
+28
Help:Wikitext examples
Created page with 'from http://en.wikipedia.org/w/index.php?title=Help:Wikitext_examples For basic information see Help:Editing. == Basic text formatting == You can format the page using Wikit...'
Restriction Enzyme Based Cloning - Ordering Primers
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m-2
Restriction Enzyme Based Cloning - Ordering Primers
no edit summary
m-3
Restriction Enzyme Based Cloning - Ordering Primers
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mRestriction Enzyme Based Cloning - Ordering Primers
added ncbi primer blast
+76
2 May 2009
Differentiation of 3T3-L1 Cells
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+41
Bridges Lab Protocols:About
Created page with 'This page contains protocols developed by and for use in the laboratory of Dr. Alan Saltiel.'
Main Page
no edit summary
+58
Restriction Enzyme Based Cloning - Ordering Primers
Created page with '==Preparation and Ordering Primers== #Choose target vector and region of interest in gene #Pick 5' and 3' restriction sites that are present in the vector but NOT in the insert #...'
Western Blotting
Protocol
+32
Western Blotting
Materials
+4
Main Page
no edit summary
+22
Western Blotting
Created page with '==Materials== *Transfer Buffer (100 mL Methanol, 50 mL 10X Transfer Buffer to final 500 mL) *Transfer Apparatus, either Bio-Rad or Invitrogen ==Protocol== #Run SDS-PAGE gel and ...'
Main Page
Cloning and Molecular Biology
+18
TOPO Cloning
Created page with '#PCR desired insert using Pfu polymerase. Add 1 uL of Taq and mix. Incubate 10min at 72C to add A to 3’ ends. Use immediately #If PCR product is a single band, no need to pu...'
Main Page
no edit summary
+60
Surface Plasmon Resonance - Protein Lipid Interactions
no edit summary
+41
Surface Plasmon Resonance - Protein Lipid Interactions
Created page with '==Materials== *L1 Sensor Chip (Biacore) *Dioleylphosphatidylcholine (Sigma) dissolved in 1:1 chloroform:methanol with 0.1% HCl *PIPx of interest (Avanti or Echelon) dissolved in ...'
Main Page
Cloning and Molecular Biology
+55
Restriction Enzyme Based Cloning
copied over protocol
Mutagenesis
Protocol
+13
Mutagenesis
Protocol
Mutagenesis
copied over protocol
Main Page
Cell and Tissue Culture
+23
Immunofluoresence
no edit summary
+1
Immunofluoresence
copied over protocol
1 May 2009
Main Page
Transcriptional Analysis
m+2
Main Page
Getting started: removed section
-273
Bradford Assay
copied over protocol
Main Page
no edit summary
+109
Transformation of Bacteria
copied over protocol
Differentiation of 3T3-L1 Cells
copied over page
Main Page
Protocols: added links
+81
Real Time PCR From Cell Culture
References (Saltiel Lab): added Chang et al Mol Endocrinol
+26
Help:Contents
wrote initial help page
Real Time PCR From Cell Culture
References (Saltiel Lab): added references
+78
Main Page
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m-62
Main Page
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+53
Real Time PCR From Cell Culture
no edit summary
+31
Help:Editing
Created page with 'from http://meta.wikimedia.org/wiki/Help:Editing#Wiki_markups_and_codes {{H:h|editor toc}} '''Editing''' refers to the changing of a page by a Wikimedia user. Unless certain re...'