930
edits
Changes
m
**::BamHI = BclI = BglII; **::EcoRI = MfeI = ApoI**::XbaI = AvrII = SpeI = NheI**::XhoI = SalI = PspXI
no edit summary
#If restriction sites are not available look for sites with compatible cohesive ends (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/compatible_cohesive_overhangs.asp).
#For example:
==Design primers==
#Design 3' end of primers with NCBI Primer Blast (http://www.ncbi.nlm.nih.gov/tools/primer-blast) or Primer3Plus (http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi).
