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#Pick 5' and 3' restriction sites that are present in the vector but NOT in the insert
#Check restriction sites either with NEBCutter (http://tools.neb.com/NEBcutter2/index.php)
#If restriction sites are not available look for sites with compatible cohesive ends (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/compatible_cohesive_overhangs.asp). #For example:
::BamHI = BclI = BglII;
::EcoRI = MfeI = ApoI
