Changes

#Check restriction sites either with NEBCutter (http://tools.neb.com/NEBcutter2/index.php)

#If restriction sites are not available look for sites with compatible cohesive ends (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/compatible_cohesive_overhangs.asp). For example:

::EcoRI = MfeI = ApoI

::XbaI = AvrII = SpeI = NheI