930
edits
Changes
copied over protocol
==Materials==
*BioRad Protein Assay Dye Reagent Concentrate cat#500-0006
*Disposable Plastic Cuvette
==Protocol==
#Dilute reagent 5X in water, stable for 2-3 weeks
#Pipet 1 mL into disposable plastic cuvette
#Add 1-10 uL of protein sample, cover with parafilm and mix
#Let sit 5-10 min to react
#Set spectrophotometer as follows:
##Go to protein assay then Bradford assay
##Set formula, then select more
##Set b=0.045 (or determine slope)
##Set dilution to be 1/vol (ie 0.1 for 10 uL)
##Blank then measure samples, absorbance must be less than 0.9
##Print and attach to experiment
==Reference==
<pubmed>942051</pubmed>
*BioRad Protein Assay Dye Reagent Concentrate cat#500-0006
*Disposable Plastic Cuvette
==Protocol==
#Dilute reagent 5X in water, stable for 2-3 weeks
#Pipet 1 mL into disposable plastic cuvette
#Add 1-10 uL of protein sample, cover with parafilm and mix
#Let sit 5-10 min to react
#Set spectrophotometer as follows:
##Go to protein assay then Bradford assay
##Set formula, then select more
##Set b=0.045 (or determine slope)
##Set dilution to be 1/vol (ie 0.1 for 10 uL)
##Blank then measure samples, absorbance must be less than 0.9
##Print and attach to experiment
==Reference==
<pubmed>942051</pubmed>
