Changes

Mutagenesis

No change in size, 00:29, 2 May 2009

→‎Protocol

==Protocol==

#Mix:

**##5 uL 10X Reaction Buffer**##~20 ng Template (1 uL of minprep)**##10 uL of Primer Mix**##5 uL of dNTP mix**##28 uL of water

#Add 1 uL of Pfu Ultra HF Polymerase

#Run PCR Program (DAVE-MUT)

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