Revision as of 14:09, 15 December 2009 by Davebridges (Talk | contribs) (added media and buffers to index page)
Revision as of 14:09, 15 December 2009 by Davebridges (Talk | contribs) (added media and buffers to index page)
Cell and Tissue Culture
- Splitting Cells
- Generating DMSO Stocks for Cell Culture
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Fugene Transfection of 293T/COS Cells
- Lipofectamine Mediated Knockdown
- Lipofectamine Plasmid Transfection
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Glucose Uptake Assay
- Luciferase Assay
- Inositol Labeling and Lipid Extraction
Cloning and Molecular Biology
Cell Biology
Protein Analysis
Protein Quantification
Protein Purification
Transcriptional Analysis
Mouse Protocols
Genotyping
Metabolic Measurements
Tissue Preparation
Media and Buffers
Yeast Media
YPD Media and Agar Yeast Selective Media and Agar
Buffers
Lysis Buffers
KRBH Buffer RIPA Buffer CHAPS Lysis Buffer
SDS-PAGE Buffers
SDS-PAGE Separating Gel Buffer SDS-PAGE Stacking Gel Buffer TORC1 Kinase Buffer Acrylamide Solution