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The following pages are not linked from or transcluded into other pages in Bridges Lab Protocols.

Showing below up to 145 results in range #1 to #145.

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  1. 3T3-L1 Plasma Membrane Isolation
  2. 5' RACE
  3. 50X TAE Buffer
  4. Acetate Incorporation into Lipid
  5. Actinomycin D
  6. Adding New Personnel to Animal Protocol
  7. Adipose Tissue Nuclear Isolation
  8. Affinity Purification of Proteins - FPLC
  9. Amitryptyline Treatment of Mice
  10. Amylose pull-down of glycogen-bound proteins
  11. Analysing Data on Armis or Great Lakes using Rmd Files
  12. Analysing Sequencing Results with SeqMan
  13. Analysis of FRET Images
  14. Antisense Oligo Knockdown in Mice
  15. BHB Assay
  16. Back-Crossing of Mouse Strains
  17. CPY Assay
  18. Chemical Hygiene Plan
  19. Chromatin Immunoprecipitation
  20. Chromatin Immunoprecipitation for Tissue Samples
  21. Cloning CRISPR-Cas Plasmids
  22. Connect to Network Drives
  23. Creating New R Packages
  24. Culturing MIN6 Cells
  25. Cycloheximide
  26. Data Management
  27. Deparaffinizing Histology Slides
  28. Designing Clones Using Benchling
  29. Designing siRNA for Knockdown
  30. Determining Cortisol Levels in Human Hair
  31. Determining Mitochondrial Copy Number
  32. Dexamethasone Treatment of Mice
  33. Differentiation of MEFs
  34. Dilution Formula
  35. DsRNA Mediated Knockdown of S2 Cells
  36. Finding and Ordering BAC Clones
  37. GST-EEA1 Pulldown from Yeast
  38. General Statistics
  39. Generating and Amplifying Adenoviral Constructs
  40. Generating and Displaying an Electostatic Surface
  41. Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap)
  42. Glucose Incorporation into Lipid and Glycogen
  43. Glycogen Determination from Cells
  44. Glycogen Determination from Tissues
  45. Glycogen Phosphorylase Assay
  46. GoTaq PCR Genotyping
  47. H&E Staining
  48. HPLC - Running a Sample or Series
  49. High Fat Diet Treatment
  50. How to Find a Journal Article
  51. How to design primer pairs for determining the presence of CrispR-cas induced mutations
  52. Immobilizing a Protein on a CM5 Chip for SPR
  53. Immunoprecipitation
  54. Inositol Labelling of Yeast Cells
  55. Installing Software to a UNIX Server
  56. Isolation of Mouse Embryonic Fibroblasts (MEFs)
  57. Isolation of Single Fibers for Seahorse Assays
  58. Ketone Body Tolerance Test
  59. Lipid Transfection
  60. Lipofectamine Transfection of C2C12 Cells
  61. Lipolysis Assay from Primary Adipocytes
  62. Liposome Binding Assay
  63. Main Page
  64. Maintanence of 18C Fly Stocks
  65. Mammary Gland Fat and SVF Separation
  66. Matrigel Basement Membrane Matrix
  67. Measurement of Glycolysis and Respiration Using Seahorse
  68. Measuring Autophagy in Tissue Culture Cells
  69. Measuring Cross-Sectional Area (CSA)
  70. Measuring Grip Strength
  71. Measuring Lifespan in Drosophila
  72. Measuring Phagocytosis using pHrodo Bioparticles
  73. Milk Creamatocrit
  74. NADH NBT Staining
  75. Oil red o stain for in vitro adipogenesis
  76. Ordering Barcodes for Mice
  77. Ordering Prescriptions-University of Michigan
  78. Osmotic Minipump Implantation
  79. PAS Staining
  80. PCR Amplification of DNA (KOD Polymerase)
  81. PCR Amplification with Platinum Taq High Fidelity
  82. PEI Mediated Plasmid Transfection
  83. Parsing XSQ Files
  84. Performing Drosophila Crosses
  85. Phosphate Buffered Saline
  86. Phred Quality Scores
  87. Preparation of Mouse Muscle for Cryosectioning
  88. Preparation of Protein Lysates from Cells
  89. Preparation of RNA Sample from Cell Culture
  90. Preparation of Tail Samples (for Genotyping)
  91. Preparing and Sending Tissues to Histology Core
  92. Protein-Lipid Overlay Assay
  93. Purification of GST-HA-S6K
  94. Purification of GST Fusion Proteins by GSTrap
  95. Purification of MBP Fusion Proteins
  96. Pyruvate Tolerance Test
  97. Quantification of Vacuolar Colocalization
  98. Quantification of miRNA by SYBR Green qPCR
  99. Quantifying Relative Expression from qRT-PCR
  100. RNA-seq Pipeline for Known Transcripts
  101. Rab5 FRET Assay
  102. Rapamycin Injections
  103. Rapamycin Sensitivity Assay of Yeast
  104. Rapamycin Stock Solution
  105. SHROB Genotyping
  106. SOP- Chloroform
  107. SOP - B-mercaptoethanol
  108. SOP - BVT.2733
  109. SOP - Bunsen Burners
  110. SOP - Centrifuge Safety
  111. SOP - Ethidium Bromide
  112. SREBF1 Genotyping Protocol
  113. Seahorse - Mitochondrial Stress Test
  114. Sending Samples for DNA Sequencing
  115. Sequence Alignment for Phylogenetic Analysis
  116. Serum Total Cholesterol Assay
  117. Single fiber dissociation of intact rodent muscles
  118. Spill Response Procedures for Infectious agents and Recombinant DNA
  119. Statistical Analysis
  120. Submitting Plasmids for Sequencing
  121. Surveyor Assay for Genomic DNA Mutations
  122. TAP Purification of Yeast Extracts
  123. TBST
  124. Tamoxifen Injections for Inducible Knockouts
  125. TaqMan SNP Array
  126. TaqMan miRNA Assay
  127. Taqman
  128. Thawing Culture Cells
  129. Triglyceride Assay from Tissue Culture Cells
  130. Using Bioconductor To Analyse Beadarray Data (lumi)
  131. Using GSEA for Gene Set Analyses of Transcriptional Data
  132. Using Git
  133. Using Github and Git for Code
  134. Using South To Migrate a Django Database
  135. Using the MBNI Cluster
  136. Using the echoMRI
  137. Using the pH Meter
  138. Verifying Kan Deletion Mutants from Genomic DNA
  139. Weigh-Suckle-Weigh Milk Volume Measurement
  140. Wiki new protocols
  141. Writing a Paper and Uploading Code to Github Repository
  142. Yeast Invertase Assay
  143. Yeast Sch9 Phosphorylation Assay
  144. Yeast Transfection (Small Scale)
  145. Yeast Transformation

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