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- 3T3-L1 Plasma Membrane Isolation
- 5' RACE
- 50X TAE Buffer
- Acetate Incorporation into Lipid
- Actinomycin D
- Adding New Personnel to Animal Protocol
- Adipose Tissue Nuclear Isolation
- Affinity Purification of Proteins - FPLC
- Amitryptyline Treatment of Mice
- Amylose pull-down of glycogen-bound proteins
- Analysing Data on Armis or Great Lakes using Rmd Files
- Analysing Sequencing Results with SeqMan
- Analysis of FRET Images
- Antisense Oligo Knockdown in Mice
- BHB Assay
- Back-Crossing of Mouse Strains
- CPY Assay
- Chemical Hygiene Plan
- Chromatin Immunoprecipitation
- Chromatin Immunoprecipitation for Tissue Samples
- Cloning CRISPR-Cas Plasmids
- Connect to Network Drives
- Creating New R Packages
- Culturing MIN6 Cells
- Cycloheximide
- Data Management
- Deparaffinizing Histology Slides
- Designing Clones Using Benchling
- Designing siRNA for Knockdown
- Determining Cortisol Levels in Human Hair
- Determining Mitochondrial Copy Number
- Dexamethasone Treatment of Mice
- Differentiation of MEFs
- Dilution Formula
- DsRNA Mediated Knockdown of S2 Cells
- Finding and Ordering BAC Clones
- GST-EEA1 Pulldown from Yeast
- General Statistics
- Generating and Amplifying Adenoviral Constructs
- Generating and Displaying an Electostatic Surface
- Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap)
- Glucose Incorporation into Lipid and Glycogen
- Glycogen Determination from Cells
- Glycogen Determination from Tissues
- Glycogen Phosphorylase Assay
- GoTaq PCR Genotyping
- H&E Staining
- HPLC - Running a Sample or Series
- High Fat Diet Treatment
- How to Find a Journal Article
- How to design primer pairs for determining the presence of CrispR-cas induced mutations
- Immobilizing a Protein on a CM5 Chip for SPR
- Immunoprecipitation
- Inositol Labelling of Yeast Cells
- Installing Software to a UNIX Server
- Isolation of Mouse Embryonic Fibroblasts (MEFs)
- Isolation of Single Fibers for Seahorse Assays
- Ketone Body Tolerance Test
- Lipid Transfection
- Lipofectamine Transfection of C2C12 Cells
- Lipolysis Assay from Primary Adipocytes
- Liposome Binding Assay
- Main Page
- Maintanence of 18C Fly Stocks
- Mammary Gland Fat and SVF Separation
- Matrigel Basement Membrane Matrix
- Measurement of Glycolysis and Respiration Using Seahorse
- Measuring Autophagy in Tissue Culture Cells
- Measuring Cross-Sectional Area (CSA)
- Measuring Grip Strength
- Measuring Lifespan in Drosophila
- Measuring Phagocytosis using pHrodo Bioparticles
- Milk Creamatocrit
- NADH NBT Staining
- Oil red o stain for in vitro adipogenesis
- Ordering Barcodes for Mice
- Ordering Prescriptions-University of Michigan
- Osmotic Minipump Implantation
- PAS Staining
- PCR Amplification of DNA (KOD Polymerase)
- PCR Amplification with Platinum Taq High Fidelity
- PEI Mediated Plasmid Transfection
- Parsing XSQ Files
- Performing Drosophila Crosses
- Phosphate Buffered Saline
- Phred Quality Scores
- Preparation of Mouse Muscle for Cryosectioning
- Preparation of Protein Lysates from Cells
- Preparation of RNA Sample from Cell Culture
- Preparation of Tail Samples (for Genotyping)
- Preparing and Sending Tissues to Histology Core
- Protein-Lipid Overlay Assay
- Purification of GST-HA-S6K
- Purification of GST Fusion Proteins by GSTrap
- Purification of MBP Fusion Proteins
- Pyruvate Tolerance Test
- Quantification of Vacuolar Colocalization
- Quantification of miRNA by SYBR Green qPCR
- Quantifying Relative Expression from qRT-PCR
- RNA-seq Pipeline for Known Transcripts
- Rab5 FRET Assay
- Rapamycin Injections
- Rapamycin Sensitivity Assay of Yeast
- Rapamycin Stock Solution
- SHROB Genotyping
- SOP- Chloroform
- SOP - B-mercaptoethanol
- SOP - BVT.2733
- SOP - Bunsen Burners
- SOP - Centrifuge Safety
- SOP - Ethidium Bromide
- SREBF1 Genotyping Protocol
- Seahorse - Mitochondrial Stress Test
- Sending Samples for DNA Sequencing
- Sequence Alignment for Phylogenetic Analysis
- Serum Total Cholesterol Assay
- Single fiber dissociation of intact rodent muscles
- Spill Response Procedures for Infectious agents and Recombinant DNA
- Statistical Analysis
- Submitting Plasmids for Sequencing
- Surveyor Assay for Genomic DNA Mutations
- TAP Purification of Yeast Extracts
- TBST
- Tamoxifen Injections for Inducible Knockouts
- TaqMan SNP Array
- TaqMan miRNA Assay
- Taqman
- Thawing Culture Cells
- Triglyceride Assay from Tissue Culture Cells
- Using Bioconductor To Analyse Beadarray Data (lumi)
- Using GSEA for Gene Set Analyses of Transcriptional Data
- Using Git
- Using Github and Git for Code
- Using South To Migrate a Django Database
- Using the MBNI Cluster
- Using the echoMRI
- Using the pH Meter
- Verifying Kan Deletion Mutants from Genomic DNA
- Weigh-Suckle-Weigh Milk Volume Measurement
- Wiki new protocols
- Writing a Paper and Uploading Code to Github Repository
- Yeast Invertase Assay
- Yeast Sch9 Phosphorylation Assay
- Yeast Transfection (Small Scale)
- Yeast Transformation