User contributions

8 December 2017

• Production of LentiCRISPR Viruses

  Removed table of contents

  16:20

  +10

• Production of LentiCRISPR Viruses

  Made new sections and added part about chloroquine

  16:19

  +237

• Preparation of PEI Stocks

  Added notes about testing transfection efficiency of PEI batches.

  16:16

  +327

• Production of LentiCRISPR Viruses

  Added categories

  16:13

  +147

• Production of LentiCRISPR Viruses

  Wrote initial protocol for virus production.

  16:12

• Preparation of Chloroquine Stocks

  Wrote instructions for making chloroquine

  15:47

• Preparation of PEI Stocks

  Added categories

  m

  15:42

  +147

• Preparation of PEI Stocks

  wrote instructions for making PEI stocks

  15:41

16 October 2017

• Measuring Fasting Insulin

  Updated with product details

  23:19

  +126

4 October 2017

• Thawing Culture Cells

  Formatting changes

  m

  15:05

  +99

2 August 2017

• Category:Media

  Created page with "This is for preparation of a variety of media for bacteria or yeast"

  14:32

• Seahorse XF Media

  changed category tag

  14:31

  -1

• Preparation of Orthovanadate Stocks

  changed category tag

  14:29

  -1

• ACSF Media

  changed category tag

  m

  14:28

  -1

• 50X TAE Buffer

  ‎Reagents: changed category tag

  m

  14:27

  -1

• Using the pH Meter

  wrote initial page

  14:27

31 July 2017

• Seahorse - Mitochondrial Stress Test

  removed table of contents

  12:28

  +10

20 July 2017

• BHB Assay

  wrote initial page for total ketone assay

  21:04

6 June 2017

• Isolation of Single Fibers for Seahorse Assays

  wrote initial protocol

  12:36

29 May 2017

• Category:Lab Safety

  Created page with "This page lists all protocols and SOP's related to lab safety."

  15:42

• SOP - BVT.2733

  copied OSEH findings into SOP

  15:41

22 May 2017

• Category:Seahorse

  Created page with "These are the protocols and buffers we use with the Seahorse bioanalyser."

  20:08

• Seahorse - Mitochondrial Stress Test

  Wrote initial page for seahorse assay

  20:06

  +2,216

17 May 2017

• Data Management

  Added link to Rmarkdown template

  14:59

  +177

16 May 2017

• Seahorse - Mitochondrial Stress Test

  created placeholder page

  19:33

• Data Management

  copied from pandoc generated mw file

  13:32

27 April 2017

• Pyruvate Tolerance Test

  fixed typo

  m

  16:39

  -4

• Pyruvate Tolerance Test

  Updated layout of the protocol

  16:35

  +12

• ACSF Media

  Updated KHPO4 concentration and fixed a typo

  13:21

  +105

• Category:Buffers

  Created page with "This page lists recipes for buffers used in various assays."

  12:42

• ACSF Media

  Added categories

  12:41

  +224

• Seahorse XF Media

  added categories

  12:41

  +165

• ACSF Media

  Wrote initial table for buffers

  12:39

28 March 2017

• Adipose Tissue Nuclear Isolation

  Updated protocol with more details

  11:56

  +1,400

27 March 2017

• Adipose Tissue Nuclear Isolation

  copied initial protocol from Kang

  20:41

10 March 2017

• Seahorse XF Media

  updated stock concentrations

  14:07

  +332

1 March 2017

• Quantification of miRNA by SYBR Green qPCR

  updated with some clarifications

  15:24

  +328

27 February 2017

• Luciferase Assay

  Changed catalog number

  m

  14:30

• Luciferase Assay

  no edit summary

  14:30

  -115

• Talk:Luciferase Assay

  copied older protocol over to talk page

  14:18

22 February 2017

• Seahorse XF Media

  Wrote initial page

  21:07

• QPCR

  Updated with smaller sample volumes

  20:16

  -174

3 February 2017

• Preparing and Sending Tissues to Histology Core

  Fixed broken link

  13:36

  -5

• Preparing and Sending Tissues to Histology Core

  Added link to histology form

  13:35

  +80

• File:UMCCC Histology Request Form.pdf

  This form is for submitting files to the UMCCC Histology Core. Enclose it in the pouch of the bag with your samples when dropping it off.

  13:31

29 January 2017

• Set up qPCR Run on Thermo Cloud and QuantStudio

  Wrote information for uploading plate to thermo cloud.

  19:35

• Set up qPCR Run on Roche LightCycler

  added categories

  18:59

  +133

• QPCR

  added categories

  m

  18:59

  +46

• Set up qPCR Run on Roche LightCycler

  Copied instructions from qPCR page

  18:58

• QPCR

  ‎Real Time qPCR: Added links to separate pages for setting up qPCR runs

  18:57

  -379

• Bridges Lab Protocols

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