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edits
Changes
Wrote information for uploading plate to thermo cloud.
==Set Up Plate Layout==
* Copy a plate layout file (example here) and save it as a CSV file.
** For each well you are using you need to enter the following:
*** Sample Name
*** Target Name
*** Task (Usually UNKNOWN)
*** Reporter (SYBR)
*** Quencher (NFQ-MGB)
** Leave the rest of the plate blank
* Log into Thermo Cloud ([https://apps.thermofisher.com/apps/dashboard/#/ https://apps.thermofisher.com/apps/dashboard] using the login information for your lab (the Bridges Lab account data is located on the side of the machine.)
* Under '''My Apps''' on the right hand side click on '''Design and Analysis Application (qPCR)'''.
* Click on '''New''' under '''Create new experiment'''.
* Leave the file name as the default (Date/ID)
** Change '''Block type''' to '''384-Well Block'''.
** Change '''Chemistry''' to '''SYBR Green Reagents'''.
** Click Next or on the '''Method''' tab and change volume to 10 uL (or leave at 20 uL if you are using a total volume of 20uL/well)
** Click Next or on the '''Plate''' tab. Click on '''Actions''' then '''Import plate setup''' and select your plate layout file. Check the visual representation of your plate layout and make sure it is correct.
** Click Next or on the '''Run''' tab. The software will save your plate layout and upload it to thermo cloud.
[[ Category: Molecular Biology ]]
[[ Category: RNA ]]
[[ Category: qPCR ]]
[[ Category: Transcription ]]
[[ Category: Expression ]]
[[ Category: Equipment Protocols ]]
* Copy a plate layout file (example here) and save it as a CSV file.
** For each well you are using you need to enter the following:
*** Sample Name
*** Target Name
*** Task (Usually UNKNOWN)
*** Reporter (SYBR)
*** Quencher (NFQ-MGB)
** Leave the rest of the plate blank
* Log into Thermo Cloud ([https://apps.thermofisher.com/apps/dashboard/#/ https://apps.thermofisher.com/apps/dashboard] using the login information for your lab (the Bridges Lab account data is located on the side of the machine.)
* Under '''My Apps''' on the right hand side click on '''Design and Analysis Application (qPCR)'''.
* Click on '''New''' under '''Create new experiment'''.
* Leave the file name as the default (Date/ID)
** Change '''Block type''' to '''384-Well Block'''.
** Change '''Chemistry''' to '''SYBR Green Reagents'''.
** Click Next or on the '''Method''' tab and change volume to 10 uL (or leave at 20 uL if you are using a total volume of 20uL/well)
** Click Next or on the '''Plate''' tab. Click on '''Actions''' then '''Import plate setup''' and select your plate layout file. Check the visual representation of your plate layout and make sure it is correct.
** Click Next or on the '''Run''' tab. The software will save your plate layout and upload it to thermo cloud.
[[ Category: Molecular Biology ]]
[[ Category: RNA ]]
[[ Category: qPCR ]]
[[ Category: Transcription ]]
[[ Category: Expression ]]
[[ Category: Equipment Protocols ]]
