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==Set Up Plate Layout==
* Log into Thermo Cloud ([https://apps.thermofisher.com/apps/dashboard/#/ https://apps.thermofisher.com/apps/dashboard] using the login information for your lab (the Bridges Lab account data is located on the side of the machine.)* Under '''My Apps''' on the right hand side click on '''Design and Analysis Application (qPCR)'''.* Copy a plate layout file (example hereclick '''Plate''' and choose '''Export plate setup''' from the '''Action''' menu) and save it as a CSV file.
** For each well you are using you need to enter the following:
*** Sample Name
*** Quencher (NFQ-MGB)
** Leave the rest of the plate blank
* Click on '''New''' under '''Create new experiment'''.
* Leave the file name as the default (Date/ID)
