915
edits
Changes
Updated KHPO4 concentration and fixed a typo
This media stock is the media for both the mitochondrial stress assays for skeletal muscle fibers. Because it is fairly well buffered, ECAR results are not reliable.
This recipe and protocol for isolating/measuring muscle fibers is found [http://www.agilent.com/cs/pubimages/misc/TB_Intact_Muscle_Fibers.pdf here] Recheck the pH after warming and prior to each use. These volumes are to make '''500 mL''' of media:
{| class="wikitable"
| 3.5 mM
|-
| Calcium Chloridechloride
| 1 M
| 650 uL
| 1.3 mM
|-
| Potassium phosphate
| 1M0.2 M| 200 uL1 mL
| 0.4 mM
|-
