'''==Materials'''==* Glucometer - AccuChek Advantage* Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [here]* Scale* Beaker for weighing mice* Syringes* 2.5g pyruvate in 10 mL PBS- This will correspond to 2.5 g/kg injections. * Timer
Glucometer - AccuChek Advantage Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [here] Scale Beaker for weighing mice Syringes 2.5g pyruvate in 10 mL PBS- This will correspond to 2.5 g/kg injections. Timer '''==Protocol''' ==* Remove food from mice for about 6h. * Typically starve the mice at 9AM and aim to start injections at 3 PM * Weigh mice, mark tails if necessary and [[take fasting glucose measurement]] via a tail clip. * Prepare pyruvate syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). * At approximately 1 min intervals, inject appropriate amount of pyruvate into interperitoneal cavity of the mouse. * Immobilize mouse and restrain tail with one hand * Aim needle between the midline and the hip bone * Insert syringe (do not inject) into cavity * Eject syringe. * At desired intervals (normally 15, 30, 45, 60, 75, 90, 105 and 120 min), take blood glucose measurements from tail vein. If needed re-snip the tail vein. When measuring glucose just lift the tail of the mouse, while leaving it in the cage, rather than removing and restraining the mouse. * Analyse data by both % change from fasting glucose and absolute values.
