Page history
10 May 2018
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+48
→Prior to starting this section:
+6
→Reverse Crosslinks of Protein/DNA Complexes to Free DNA
+18
10 January 2018
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+2
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+122
9 January 2018
→Prior to starting this section:
-8
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+6
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+2
→Before You Start
+57
→Buffers and Solutions Needed
+22
→Buffers and Solutions Needed
+139
→Buffers and Solutions Needed
-79
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
→Prior to starting this section:
-11
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+3
→Reverse Crosslinks of Protein/DNA Complexes to Free DNA
+6
→Prior to starting this section:
-1
→Immunoprecipitation
+16
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+6
→Crosslinking, Lysis and Shearing of DNA
+19
→Immunoprecipitation
+58
no edit summary
-4
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
-32
→Immunoprecipitation
+3,234
29 June 2017
12 June 2017
21 August 2016
10 June 2016
23 March 2016
→Incubate bead-antibody complex with fragmented, cross-linked chromatin
+1
→Incubate bead-antibody complex with fragmented, cross-linked chromatin
+46
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+16
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+255
→Purification of ChIP DNA
+24
21 March 2016
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
-20
→Incubate bead-antibody complex with fragmented, cross-linked chromatin
-11
→Reverse cross-linking and recover ChIP DNA
-15
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+3
→Buffers and Solutions Needed
+32
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+6
→Incubate bead-antibody complex with fragmented, cross-linked chromatin
-4
→Reverse cross-linking and recover ChIP DNA
+6
→Analysis of Immunoprecipitated DNA
-25
→Reverse cross-linking and recover ChIP DNA
+1,117
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+184
→Crosslinking, Lysis and Shearing of DNA
+76
→Crosslinking, Lysis and Shearing of DNA
+22