Search results

Create the page "PCR" on this wiki! See also the search results found.

Page title matches

• Real Time PCR From Cell Culture
  *SyberGreen PCR Master Mix Applied Biosystems ===RT-PCR Reaction===
  3 KB (423 words) - 00:42, 29 March 2011
• PCR Amplification of DNA
  *DreamTaq Green PCR Master mix - contains dNTPs, polymerase, salts, and buffer with loading dye ==PCR Program==
  1 KB (162 words) - 16:57, 8 June 2020
• PCR Analysis of Tail DNA
  Run "specfic" PCR Program for gene of interest (approx 2 hours). *[[PCR Amplification of DNA]]
  773 bytes (111 words) - 17:00, 8 June 2020
• Colony PCR
  #Prepare PCR: ===Run PCR Program Colony PCR:===
  690 bytes (108 words) - 15:43, 21 October 2009
• PCR Amplification of DNA (KOD Polymerase)
  *Run PCR Program (approx 3.5 to 4 hours). Normally use touchdown PCR ('''TD-KOD''') as follows: *Purify PCR product if necessary using Qiagen kit (Add 5x PB)
  1 KB (179 words) - 21:24, 3 November 2009
• GoTaq PCR Genotyping
  *Prepare gel 30 minutes before PCR is finished. *Add this mixture to each PCR tube.
  1 KB (134 words) - 14:50, 6 December 2010
• Cre PCR
  ==PCR Program== [[ Category:PCR]]
  301 bytes (43 words) - 18:42, 25 April 2018
• Quantifying Relative Expression from qRT-PCR
  2 KB (250 words) - 14:57, 2 December 2013
• PCR Amplification with Platinum Taq High Fidelity
  * Platinum PCR SuperMix High Fidelity (Life Technologies cat# 12532-016) * The PCR program should be
  745 bytes (105 words) - 13:43, 17 July 2015
• BDNF PCR
  491 bytes (80 words) - 14:13, 1 April 2014
• RT-PCR primer design for ChIP
  # PCR product size should be set to 70-150bp. [[ Category: PCR ]]
  1 KB (224 words) - 14:24, 21 August 2016

Page text matches

• Real Time PCR From Cell Culture
  *SyberGreen PCR Master Mix Applied Biosystems ===RT-PCR Reaction===
  3 KB (423 words) - 00:42, 29 March 2011
• Main Page
  *[[PCR Amplification of DNA]] *[[Real Time PCR From Cell Culture]]
  4 KB (484 words) - 12:10, 15 August 2016
• Mutagenesis
  #Run PCR Program (Mutagenesis 8/15/20 minute, depending on template)
  1 KB (208 words) - 15:15, 13 January 2010
• Restriction Enzyme Based Cloning
  #If insert is PCR, run PCR then PCR purify (Qiagen kit). Elute in 30 uL EB
  1 KB (227 words) - 14:40, 7 May 2009
• TOPO Cloning
  #PCR desired insert using Pfu polymerase. Add 1 uL of Taq and mix. Incubate 10 #If PCR product is a single band, no need to purify further. If several bands, gel
  611 bytes (109 words) - 00:45, 2 May 2009
• PCR Amplification of DNA
  *DreamTaq Green PCR Master mix - contains dNTPs, polymerase, salts, and buffer with loading dye ==PCR Program==
  1 KB (162 words) - 16:57, 8 June 2020
• PCR Analysis of Tail DNA
  Run "specfic" PCR Program for gene of interest (approx 2 hours). *[[PCR Amplification of DNA]]
  773 bytes (111 words) - 17:00, 8 June 2020
• QPCR - Lin Lab
  ==PCR Program:== Protocol written for 96-well Real-time PCR machine in Jiandie Lin lab on 10/4/2006.
  3 KB (562 words) - 12:13, 28 May 2009
• Designing and Preparing dsRNA
  *set up a 250 uL PCR reaction **15 uL MgCl (this can be adjusted to optimize PCR conditions)
  2 KB (290 words) - 14:31, 1 October 2009
• Colony PCR
  #Prepare PCR: ===Run PCR Program Colony PCR:===
  690 bytes (108 words) - 15:43, 21 October 2009
• PCR Amplification of DNA (KOD Polymerase)
  *Run PCR Program (approx 3.5 to 4 hours). Normally use touchdown PCR ('''TD-KOD''') as follows: *Purify PCR product if necessary using Qiagen kit (Add 5x PB)
  1 KB (179 words) - 21:24, 3 November 2009
• GoTaq PCR Genotyping
  *Prepare gel 30 minutes before PCR is finished. *Add this mixture to each PCR tube.
  1 KB (134 words) - 14:50, 6 December 2010
• Preparing an Adenoviral shRNA Clone
  ...ligo + 48 uL annealing buffer (100 mM NaCl and 50 mM HEPES pH 7.4.) into a PCR tube.
  2 KB (353 words) - 19:47, 13 December 2010
• Genotyping Program
  |[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || aTSC, mTSC, Placenta TSC || Two bands if heter |[[Cre Genotyping|Cre]] || [[Cre PCR ]] || Between 340-400kDa || aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta
  2 KB (297 words) - 14:32, 23 July 2021
• Preparation of Tail Samples (for Genotyping)
  ...Proteinase K and mouse tail in an eppendorf tube or in a well of a 96 well PCR plate (Proteinase K stock = 10mg/mL in ddH2O)
  1 KB (184 words) - 15:20, 21 May 2018
• Rapid Isolation of Genomic DNA from Yeast
  [[Category:PCR]]
  1 KB (197 words) - 16:32, 21 February 2012
• Verifying Kan Deletion Mutants from Genomic DNA
  [[Category:PCR]] ...[[Rapid Isolation of Genomic DNA from Yeast]]. Use 1µl of the DNA in your PCR reaction.
  1 KB (248 words) - 16:39, 21 February 2012
• CAP Genotyping
  [[Category: PCR ]] *Get sequences from Xiao-ling, stocks in XL PCR Box
  281 bytes (35 words) - 17:26, 27 February 2012
• First Strand cDNA Synthesis (AB Kit)
  * Run the PCR Program '''AB Reaction Protocol'''. Takes ~2h:
  1 KB (154 words) - 15:16, 24 July 2019
• Vinexin Genotyping
  [[ Category:PCR]] ==PCR Program==
  2 KB (225 words) - 15:14, 6 August 2012
• Isolation of Mouse Embryonic Fibroblasts (MEFs)
  # Disect out all four limbs plus the tail. Place one small piece into PCR tube/plate for genotyping
  741 bytes (117 words) - 15:50, 5 September 2012
• Ob/ob Genotyping
  ==PCR Reaction== The PCR program is called '''ob-ob''' and is:
  1 KB (195 words) - 19:59, 26 September 2013
• 5' RACE
  <li> Combine the following in a PCR tube:</li> <li> Incubate at 70C for 10 min to denature RNA using the PCR Program '''5 RACE 70C'''</li>
  3 KB (551 words) - 21:04, 4 December 2012
• TSC-floxed Genotyping
  ...sc1 and the deleted allele using the following three primers in a 35 cycle PCR reaction using Perkin Elmer AmpliTaq Gold: F4536, 5′-AGGAGGCCTCTTCTGCTACC [[ Category:PCR]]
  1 KB (141 words) - 19:11, 30 December 2012
• MTM1 Genotyping
  [[ Category:PCR]] ==PCR Program==
  663 bytes (93 words) - 15:17, 21 January 2013
• TC10b Genotyping
  [[ Category:PCR]]
  495 bytes (59 words) - 14:55, 5 July 2013
• Cre PCR
  ==PCR Program== [[ Category:PCR]]
  301 bytes (43 words) - 18:42, 25 April 2018
• Primer Design for qPCR
  # Under PCR Product Size pick 70-150 as the range ...opriate primer pair in that region is not selected, change the range under PCR template on the top
  1 KB (249 words) - 17:48, 29 June 2017
• QPCR
  *SYBR Green PCR Master Mix Applied Biosystems (ThermoFisher Catalog # 4367659; [https://www
  4 KB (585 words) - 19:13, 8 November 2023
• Cloning CRISPR-Cas Plasmids
  * First digest vector by adding to a PCR tube (or use a frozen, pre-digested vector): * Next prepare the insert by annealing and phosphorylating the primers in a PCR tube:
  2 KB (324 words) - 19:41, 19 October 2017
• PCR Amplification with Platinum Taq High Fidelity
  * Platinum PCR SuperMix High Fidelity (Life Technologies cat# 12532-016) * The PCR program should be
  745 bytes (105 words) - 13:43, 17 July 2015
• TaqMan miRNA Assay
  ...w.lifetechnologies.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays.html * You will then prepare a tube for each primer/RNA set as such in a PCR tube:
  1 KB (234 words) - 18:52, 30 December 2014
• Surveyor Assay for Genomic DNA Mutations
  * Platinum PCR SuperMix High Fidelity (Life Technologies cat# 12532-016) * Place in PCR tube and digest using '''quick-extract''' PCR program:
  2 KB (262 words) - 20:02, 13 July 2015
• Quantification of miRNA by SYBR Green qPCR
  * Briefly centrifuge then transfer to a PCR tube and add (per reaction): * Mix gently by pipetting and place in the PCR machine for the following program (called '''SS III Reaction'''):
  3 KB (432 words) - 15:24, 1 March 2017
• TaqMan SNP Assay
  *StepOnePlus Real-Time PCR System (Applied Biosystems).
  1 KB (155 words) - 16:39, 16 November 2015
• SHROB Genotyping
  ...fragments of 82 bp and 39 bp in size (lanes 6-1 0). Tru9l digestion of the PCR products from heterozygous lean Koletsky (+/fa rats gave rise to three 121- [[ Category:PCR]]
  3 KB (371 words) - 17:09, 8 June 2020
• Chromatin Immunoprecipitation
  * QIAquick PCR Purification Kit 39. Add 5 volumes Qiagen Buffer PB (QIAquick PCR Purification Kit) to one volume of ChIP’d DNA. Add pH detector (at a 1:25
  11 KB (1,742 words) - 15:23, 10 May 2018
• RT-PCR primer design for ChIP
  # PCR product size should be set to 70-150bp. [[ Category: PCR ]]
  1 KB (224 words) - 14:24, 21 August 2016
• Locating ChIP-seq peaks from ENCODE
  # Design primers for genes you believe your protein is bound to [[RT-PCR primer design for ChIP]]
  1 KB (272 words) - 17:15, 25 January 2016
• Chromatin Immunoprecipitation for Tissue Samples
  # Add 5 volumes Qiagen Buffer PB (QIAquick PCR Purification Kit) to one volume of ChIP’d DNA. Add pH detector (at a 1:25 # Add half (~600 µl) of the solution to a QIAquick PCR Purification column, centrifuge for 30-60 sec @ 13,000 RPM , and then repea
  6 KB (1,077 words) - 21:43, 10 June 2016
• SREBF1 Genotyping Protocol
  ==PCR Reaction== The PCR program is called "srebp" on the thermal cycler and is as follows:
  1 KB (184 words) - 18:27, 30 August 2016
• Nr3c1 Genotyping
  ==PCR PROTOCOL== [[ Category:PCR]]
  585 bytes (80 words) - 19:00, 17 November 2020
• Cre Genotyping
  [[ Category:PCR]]
  264 bytes (33 words) - 15:14, 5 June 2020
• Mc4r-Cone Genotyping
  Make primers at 1uM (Fwd + Rev and Fwd + Neo Rev). PCR primers separately. ==PCR PROTOCOL==
  815 bytes (103 words) - 17:54, 5 June 2020
• Ppp1r3c Genotyping
  Make primers at 1uM (Fwd + Rev and Fwd + Neo Rev}. PCR primers separately. ==PCR PROTOCOL==
  668 bytes (65 words) - 18:17, 5 June 2020
• 4EBP1/2 Genotyping
  ...1uM (Fwd1 + Rev1, Neo Fwd1/2 + Rev1, Fwd2 + Rev2, and Neo Fwd1/2 + Rev2). PCR 4EBP1 and 4EBP2 primers separately. [[ Category:PCR]]
  639 bytes (116 words) - 14:41, 8 June 2020
• GDF15 Genotyping
  Make primers at 0.4uM (Fwd1 + Rev and Fwd2 + Rev). PCR primers separately. ==PCR PROTOCOL==
  705 bytes (86 words) - 14:51, 8 June 2020