User contributions
This user is currently blocked. The latest block log entry is provided below for reference:
- 14:32, 23 July 2021 (diff | hist) . . (+19) . . Genotyping Program (added are band size) (current)
- 14:31, 23 July 2021 (diff | hist) . . (+128) . . Genotyping Program (Added SRY)
- 14:30, 23 July 2021 (diff | hist) . . (+130) . . N SRY Genotyping (Create SRY) (current)
- 17:42, 8 April 2021 (diff | hist) . . (-1) . . Weigh-Suckle-Weigh Milk Volume Measurement (current)
- 15:58, 26 March 2021 (diff | hist) . . (+14) . . Western Blotting (current)
- 15:51, 26 March 2021 (diff | hist) . . (-1) . . Scanning and Analyzing Western Blots Using LiCor Odyssey (→Analysis/Quantification) (current)
- 15:51, 26 March 2021 (diff | hist) . . (+6) . . Scanning and Analyzing Western Blots Using LiCor Odyssey (→Analysis/Quantification)
- 15:50, 26 March 2021 (diff | hist) . . (+5,230) . . N Scanning and Analyzing Western Blots Using LiCor Odyssey (protocol on how to use the licor)
- 13:54, 26 March 2021 (diff | hist) . . (-145) . . Western Blotting (removed ECL instructions- JR)
- 19:00, 17 November 2020 (diff | hist) . . (+167) . . Nr3c1 Genotyping (current)
- 17:10, 8 June 2020 (diff | hist) . . (+99) . . Tamoxifen Injections for Inducible Knockouts (added SOPs) (current)
- 17:09, 8 June 2020 (diff | hist) . . (+40) . . SHROB Genotyping (added SOPs) (current)
- 17:07, 8 June 2020 (diff | hist) . . (+88) . . Rapamycin Injections (added SOPs) (current)
- 17:05, 8 June 2020 (diff | hist) . . (+181) . . Perfusion of Mouse (added SOPs) (current)
- 17:02, 8 June 2020 (diff | hist) . . (+132) . . Paraffin Embedding of Tissue Samples (added SOPs)
- 17:00, 8 June 2020 (diff | hist) . . (+2) . . PCR Analysis of Tail DNA (current)
- 17:00, 8 June 2020 (diff | hist) . . (+51) . . PCR Analysis of Tail DNA
- 16:59, 8 June 2020 (diff | hist) . . (0) . . PCR Analysis of Tail DNA
- 16:57, 8 June 2020 (diff | hist) . . (+1) . . PCR Amplification of DNA (current)
- 16:56, 8 June 2020 (diff | hist) . . (-73) . . PCR Amplification of DNA (updated)
- 16:49, 8 June 2020 (diff | hist) . . (+20) . . PCR Amplification of DNA (updated)
- 15:34, 8 June 2020 (diff | hist) . . (+68) . . PAS Staining (added SOPs) (current)
- 15:32, 8 June 2020 (diff | hist) . . (+71) . . Osmotic Minipump Implantation (current)
- 15:29, 8 June 2020 (diff | hist) . . (+68) . . NADH NBT Staining (added SOPs) (current)
- 15:28, 8 June 2020 (diff | hist) . . (+29) . . Making LB Agar Plates (added SOPs) (current)
- 15:25, 8 June 2020 (diff | hist) . . (+67) . . Insulin Tolerance Test (added SOPS)
- 15:23, 8 June 2020 (diff | hist) . . (+89) . . H&E Staining (added SOPs) (current)
- 15:05, 8 June 2020 (diff | hist) . . (+68) . . Glucose Tolerance Test (added SOP)
- 15:00, 8 June 2020 (diff | hist) . . (+18) . . Genotyping Program
- 14:59, 8 June 2020 (diff | hist) . . (+267) . . Genotyping Program (added relevant strains)
- 14:51, 8 June 2020 (diff | hist) . . (+705) . . N GDF15 Genotyping (created page) (current)
- 14:47, 8 June 2020 (diff | hist) . . (+2) . . Genotyping Program
- 14:46, 8 June 2020 (diff | hist) . . (+119) . . Genotyping Program (added gdf15)
- 14:41, 8 June 2020 (diff | hist) . . (+44) . . 4EBP1/2 Genotyping (added band size) (current)
- 14:39, 8 June 2020 (diff | hist) . . (+9) . . 4EBP1/2 Genotyping
- 14:38, 8 June 2020 (diff | hist) . . (-6) . . 4EBP1/2 Genotyping
- 14:35, 8 June 2020 (diff | hist) . . (+4) . . 4EBP1/2 Genotyping
- 14:35, 8 June 2020 (diff | hist) . . (+16) . . 4EBP1/2 Genotyping
- 14:35, 8 June 2020 (diff | hist) . . (+572) . . N 4EBP1/2 Genotyping (created page)
- 14:10, 8 June 2020 (diff | hist) . . (+191) . . Genotyping Program (added 4ebp1/2)
- 18:17, 5 June 2020 (diff | hist) . . (+668) . . N Ppp1r3c Genotyping (created page) (current)
- 17:57, 5 June 2020 (diff | hist) . . (+124) . . Genotyping Program (added PTG)
- 17:54, 5 June 2020 (diff | hist) . . (+121) . . Mc4r-Cone Genotyping (added original google doc) (current)
- 17:49, 5 June 2020 (diff | hist) . . (+6) . . Mc4r-Cone Genotyping
- 17:48, 5 June 2020 (diff | hist) . . (+688) . . N Mc4r-Cone Genotyping (create page)
- 17:41, 5 June 2020 (diff | hist) . . (+1) . . Genotyping Program
- 15:21, 5 June 2020 (diff | hist) . . (+149) . . Genotyping Program (added mc4r-Cone)
- 15:14, 5 June 2020 (diff | hist) . . (+264) . . N Cre Genotyping (created page) (current)
- 15:10, 5 June 2020 (diff | hist) . . (+112) . . Nr3c1 Genotyping (added cre primers)
- 15:08, 5 June 2020 (diff | hist) . . (+120) . . Nr3c1 Genotyping
- 15:07, 5 June 2020 (diff | hist) . . (+2) . . Nr3c1 Genotyping
- 15:07, 5 June 2020 (diff | hist) . . (+2) . . Nr3c1 Genotyping
- 15:06, 5 June 2020 (diff | hist) . . (+54) . . Nr3c1 Genotyping
- 15:04, 5 June 2020 (diff | hist) . . (+128) . . N Nr3c1 Genotyping (created page)
- 14:46, 5 June 2020 (diff | hist) . . (+66) . . Genotyping Program (added newer genotyping strains)
- 14:43, 5 June 2020 (diff | hist) . . (+24) . . Genotyping Program
- 14:28, 5 June 2020 (diff | hist) . . (+79) . . Freezing and Thawing S2 Cells (added SOP requirements-JR) (current)
- 21:56, 4 June 2020 (diff | hist) . . (+87) . . Differentiation of 3T3-L1 Cells (added SOP requirements) (current)
- 21:52, 4 June 2020 (diff | hist) . . (+92) . . Deparaffinizing Histology Slides (added SOP requirements) (current)
- 16:38, 4 June 2020 (diff | hist) . . (+35) . . Preparing a SDS-PAGE Gel (added SOP requirement-JR) (current)
- 18:05, 13 March 2020 (diff | hist) . . (+710) . . N How to Find a Journal Article (Created page with "#Check the recent '''tables of contents''' for journals we discuss from regularly, like '''Cell Metabolism''', '''Diabetes''', '''Molecular Metabolism''', '''Cell''', '''Natur...") (current)
- 19:20, 17 January 2020 (diff | hist) . . (+61) . . Splitting Cells (added SOP hyperlinks) (current)
- 19:19, 17 January 2020 (diff | hist) . . (+32) . . Splitting Cells
- 15:14, 12 September 2019 (diff | hist) . . (+17,284) . . N SOP - Corrosives (Created page with " Category: SOP Category: Lab Safety '''Laboratory Director (LD) Approval is Required Prior to Performing this Procedure ''' ==Description== This standard operating...")
- 15:06, 12 September 2019 (diff | hist) . . (+48) . . SOP - Tamoxifen (JRR signed)
- 15:03, 12 September 2019 (diff | hist) . . (+48) . . SOP - Cytotoxins and Antineoplastic Drugs (JRR signed)
- 15:02, 12 September 2019 (diff | hist) . . (+48) . . SOP - Cryogenic Materials (JRR signed)
- 14:56, 12 September 2019 (diff | hist) . . (+49) . . SOP - Bunsen Burners (JRR signed)
- 14:55, 12 September 2019 (diff | hist) . . (0) . . SOP - B-mercaptoethanol
- 14:54, 12 September 2019 (diff | hist) . . (+49) . . SOP - B-mercaptoethanol (JRR signed)
- 16:06, 25 April 2019 (diff | hist) . . (+52) . . Preparation of Protein Lysates from Mouse Tissues (added different RIPA volume for WAT) (current)
- 17:51, 12 February 2019 (diff | hist) . . (+118) . . Safety and Animal Training
- 14:54, 8 January 2019 (diff | hist) . . (+2) . . SOP - Animal Anesthetics
- 14:40, 8 January 2019 (diff | hist) . . (+63) . . Splitting Cells
- 14:37, 8 January 2019 (diff | hist) . . (0) . . Western Blotting
- 20:30, 16 July 2018 (diff | hist) . . (+697) . . PAS Staining
- 20:22, 16 July 2018 (diff | hist) . . (0) . . Deparaffinizing Histology Slides
- 20:20, 16 July 2018 (diff | hist) . . (+402) . . N Deparaffinizing Histology Slides (created)
- 15:20, 21 May 2018 (diff | hist) . . (+2) . . Preparation of Tail Samples (for Genotyping) (changed g to ml for detergents) (current)
- 17:06, 9 April 2018 (diff | hist) . . (+21) . . Preparation of Protein Lysates from Mouse Tissues
- 20:15, 22 March 2018 (diff | hist) . . (+128) . . N Chemical Hygiene Plan (Create Protocol-Online link to Chemical Hygiene Plan) (current)
- 20:20, 15 March 2018 (diff | hist) . . (+12) . . m Preparation of Protein Lysates from Mouse Tissues
- 14:39, 15 March 2018 (diff | hist) . . (+29) . . m Bradford Assay (current)
- 14:37, 15 March 2018 (diff | hist) . . (+144) . . Bradford Assay (Adjusted bradford reagent vol and added standard curve details)
- 14:27, 15 March 2018 (diff | hist) . . (+1,021) . . N Ordering Prescriptions-University of Michigan (Created protocol) (current)
- 14:21, 15 March 2018 (diff | hist) . . (+1) . . Set up qPCR Run on Thermo Cloud and QuantStudio
- 15:41, 26 February 2018 (diff | hist) . . (+21) . . Western Blotting
- 17:01, 22 February 2018 (diff | hist) . . (+2,022) . . N QPCR Analysis on ThermoCloud (Create Protocol)
- 21:13, 21 February 2018 (diff | hist) . . (+1) . . m Set up qPCR Run on Thermo Cloud and QuantStudio
- 21:09, 21 February 2018 (diff | hist) . . (+590) . . Set up qPCR Run on Thermo Cloud and QuantStudio (added detail)
- 20:46, 21 February 2018 (diff | hist) . . (+69) . . Set up qPCR Run on Thermo Cloud and QuantStudio (Added detail)
- 16:29, 14 February 2018 (diff | hist) . . (0) . . QPCR
- 20:10, 2 November 2017 (diff | hist) . . (+121) . . Preparation of Protein Lysates from Mouse Tissues (→Protocol)
- 16:59, 12 October 2017 (diff | hist) . . (+55) . . Safety and Animal Training (added bbp)
- 16:51, 12 October 2017 (diff | hist) . . (+78) . . SOP - Biosafety Cabinets (JR Signed off)
- 16:14, 29 September 2017 (diff | hist) . . (+40) . . Safety and Animal Training (added category-JR)
- 14:01, 17 August 2017 (diff | hist) . . (-1) . . Preparation of Tail Samples (for Genotyping) (→PBND Solution: Tail Lysis Buffer)
- 14:01, 17 August 2017 (diff | hist) . . (-1) . . Preparation of Tail Samples (for Genotyping)
- 14:00, 17 August 2017 (diff | hist) . . (-6) . . Preparation of Tail Samples (for Genotyping) (→PBND Solution: Tail Lysis Buffer)
- 13:58, 17 August 2017 (diff | hist) . . (+1) . . Preparation of Tail Samples (for Genotyping) (→PBND Solution: Tail Lysis Buffer)
- 13:56, 17 August 2017 (diff | hist) . . (+11) . . Preparation of Tail Samples (for Genotyping)
- 13:54, 17 August 2017 (diff | hist) . . (+660) . . Preparation of Tail Samples (for Genotyping) (→PBND Solution: Tail Lysis Buffer)
- 17:26, 15 June 2017 (diff | hist) . . (+76) . . Spill Response Procedures for Infectious agents and Recombinant DNA
- 14:47, 13 June 2017 (diff | hist) . . (+75) . . SOP - Phenol (JR Signed off)
- 14:36, 9 June 2017 (diff | hist) . . (+45) . . SOP - Tamoxifen (Added category-JR)
- 14:36, 9 June 2017 (diff | hist) . . (+46) . . SOP - Cryogenic Materials (Added category-JR)
- 14:34, 9 June 2017 (diff | hist) . . (+46) . . SOP - B-mercaptoethanol (Added category-JR)
- 14:33, 9 June 2017 (diff | hist) . . (+46) . . SOP - Biosafety Cabinets (Added category-JR)
- 14:31, 9 June 2017 (diff | hist) . . (+47) . . SOP - Cytotoxins and Antineoplastic Drugs (Added category-JR)
- 14:05, 9 June 2017 (diff | hist) . . (+73) . . SOP - Vacuum Pumps (JR Signed off)
- 14:05, 9 June 2017 (diff | hist) . . (+74) . . SOP - Irritants (JR Signed off)
- 14:04, 9 June 2017 (diff | hist) . . (+74) . . SOP - Compressed Gases (JR Signed off)
- 14:04, 9 June 2017 (diff | hist) . . (+74) . . SOP - Autoclave (JR Signed off)
- 14:03, 9 June 2017 (diff | hist) . . (+74) . . SOP - Needle Capping (JR Signed off)
- 14:03, 9 June 2017 (diff | hist) . . (+74) . . SOP - Flammables and Combustibles (JR Signed off)
- 14:02, 9 June 2017 (diff | hist) . . (-1) . . SOP - Ethidium Bromide
- 14:01, 9 June 2017 (diff | hist) . . (+73) . . SOP - Chloroform (JR Signed off)
- 14:01, 9 June 2017 (diff | hist) . . (+74) . . SOP - Centrifuge Safety (JR Signed off)
- 13:59, 9 June 2017 (diff | hist) . . (+74) . . Safety and Animal Training
- 15:33, 8 May 2017 (diff | hist) . . (+9) . . First Strand cDNA Synthesis (AB Kit)
- 17:44, 28 April 2017 (diff | hist) . . (+143) . . Safety and Animal Training
- 15:15, 28 April 2017 (diff | hist) . . (+155) . . Matrigel Basement Membrane Matrix (current)
- 14:18, 28 April 2017 (diff | hist) . . (+16) . . Matrigel Basement Membrane Matrix
- 14:15, 28 April 2017 (diff | hist) . . (+602) . . N Matrigel Basement Membrane Matrix (Created page with "==STORAGE== Store unopened an aliquoted Matrigel at -20°C. DO NOT STORE IN FROST-FREE FREEZER. KEEP FROZEN. ==RECONSTRUCTION AND USE== *Color variation may occur betwee...")
- 13:59, 28 April 2017 (diff | hist) . . (+42) . . Safety and Animal Training
- 13:57, 28 April 2017 (diff | hist) . . (+96) . . Safety and Animal Training
- 13:56, 28 April 2017 (diff | hist) . . (+47) . . Safety and Animal Training
- 16:55, 7 November 2016 (diff | hist) . . (+6,723) . . N SOP - Sensitizers (Created page with "Category: SOP Category: Lab Safety ==Description== This standard operating procedure outlines the handling and use of sensitizers. Review this document and supply the...")
- 16:13, 13 October 2016 (diff | hist) . . (+1) . . SOP - Rapamycin (→Exposures/Unintended Contact)
- 16:12, 13 October 2016 (diff | hist) . . (+1) . . SOP - Rapamycin (→=Exposures/Unintended Contact)
- 16:12, 13 October 2016 (diff | hist) . . (+13,170) . . N SOP - Rapamycin (Created page with " Category: SOP Category: Lab Safety ==Description== ''This standard operating procedure outlines the handling and use of rapamycin. Review this document and supply...")
- 15:59, 13 October 2016 (diff | hist) . . (0) . . SOP - Bleach (→Certification)
- 15:58, 13 October 2016 (diff | hist) . . (+2) . . SOP - Formalin and Paraformaldehyde (→Useful Formalin and Paraformaldehyde Links:)
- 15:58, 13 October 2016 (diff | hist) . . (+8,774) . . N SOP - Formalin and Paraformaldehyde (Created page with " Category: SOP Category: Lab Safety '''Laboratory Director (LD) Approval is Required Prior to Performing this Procedure''' ==Description== This standard operating...")
- 15:48, 13 October 2016 (diff | hist) . . (+8,842) . . N SOP - Ethidium Bromide (Created page with " Category: SOP Category: Lab Safety ==Description== This standard operating procedure outlines the handling and use of Ethidium Bromide (EtBr). Review this documen...")
- 15:41, 13 October 2016 (diff | hist) . . (-94) . . SOP - Bleach (→Certification)
- 15:41, 13 October 2016 (diff | hist) . . (+2) . . SOP - Bleach
- 15:01, 13 October 2016 (diff | hist) . . (+48) . . SOP - Electrophoresis
- 15:00, 13 October 2016 (diff | hist) . . (-3) . . SOP - Electrophoresis (→Exposures/Unintended Contact)
- 15:00, 13 October 2016 (diff | hist) . . (+7,454) . . N SOP - Electrophoresis (Created page with "==Description== This standard operating procedure outlines the process of electrophoresis, including the use of equipment and chemicals necessary for the process. Review this...")
- 14:49, 13 October 2016 (diff | hist) . . (+54) . . SOP - Carcinogens
- 14:45, 13 October 2016 (diff | hist) . . (+137) . . SOP - Animal Anesthetics (→Certification)
- 14:44, 13 October 2016 (diff | hist) . . (-136) . . SOP - Animal Anesthetics
- 13:33, 13 October 2016 (diff | hist) . . (0) . . SOP - Carcinogens (→Exposures/Unintended Contact)
- 13:32, 13 October 2016 (diff | hist) . . (+11,758) . . N SOP - Carcinogens (Created page with "Laboratory Director (LD) Approval is Required Prior to Performing this Procedure ==Description== This standard operating procedure is designed to provide guidance in writing...")
- 17:14, 12 October 2016 (diff | hist) . . (+10,991) . . N SOP - Animal Anesthetics (Created page with " Category: SOP Category: Lab Safety ==Description== This standard operating procedure outlines the handling and use of animal anesthetics including: isoflurane, hal...")
- 16:42, 12 October 2016 (diff | hist) . . (-9) . . SOP - Bleach
- 16:42, 12 October 2016 (diff | hist) . . (+64) . . SOP - Bleach
- 16:41, 12 October 2016 (diff | hist) . . (+24) . . SOP - Bleach
- 15:20, 9 September 2016 (diff | hist) . . (+98) . . 50X TAE Buffer
- 14:42, 9 September 2016 (diff | hist) . . (-4) . . 50X TAE Buffer
- 14:41, 9 September 2016 (diff | hist) . . (+232) . . N 50X TAE Buffer (Created page with "==Reagents== *900mL ddH2O *242g Tris Base *57.1 mL Glacial Acetic Acid *18.6g EDTA 1. Add Tris Base, Glacial Acetic Acid, and EDTA to 900mL water in 1L Erlenmeyer Flask and...")
- 18:27, 30 August 2016 (diff | hist) . . (+1) . . SREBF1 Genotyping Protocol (current)
- 18:26, 30 August 2016 (diff | hist) . . (-62) . . SREBF1 Genotyping Protocol
- 18:25, 30 August 2016 (diff | hist) . . (+62) . . SREBF1 Genotyping Protocol
- 18:10, 30 August 2016 (diff | hist) . . (0) . . N File:Screen Shot 2016-08-30 at 12.48.27 PM.png (current)
- 18:09, 30 August 2016 (diff | hist) . . (+4) . . SREBF1 Genotyping Protocol
- 18:01, 30 August 2016 (diff | hist) . . (+9) . . SREBF1 Genotyping Protocol
- 16:50, 30 August 2016 (diff | hist) . . (+41) . . SREBF1 Genotyping Protocol
- 16:32, 30 August 2016 (diff | hist) . . (+1,047) . . N SREBF1 Genotyping Protocol (Created page with "==Primers== *Srebf1 WT Fwd: 5' CCA TGT GCG CTC ACC CGA G 3' *Srebf1 All Fwd: 5' CGA TCC GCT GTA GAG ACC CT 3' *Srebf1 All REV: 5' CAT TCA GAG CAC CCG GTG AA 3' ==Primer...")