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The following pages do not link to other pages in Bridges Lab Protocols.

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  1. Mc4r-Cone Genotyping
  2. Measurement of Glycolysis and Respiration Using Seahorse
  3. Measuring Autophagy in Tissue Culture Cells
  4. Measuring Cross-Sectional Area (CSA)
  5. Measuring Grip Strength
  6. Measuring Phagocytosis using pHrodo Bioparticles
  7. Milk Creamatocrit
  8. Monitoring Food Intake
  9. Nr3c1 Genotyping
  10. Ob/ob Genotyping
  11. Oil red o stain for in vitro adipogenesis
  12. Ordering Barcodes for Mice
  13. Ordering Mice
  14. Ordering Prescriptions-University of Michigan
  15. PCR Amplification of DNA (KOD Polymerase)
  16. PCR Amplification with Platinum Taq High Fidelity
  17. Parsing XSQ Files
  18. Performing Drosophila Crosses
  19. Phosphate Buffered Saline
  20. Phred Quality Scores
  21. Power Analysis
  22. Ppp1r3c Genotyping
  23. Preparation of Chloroquine Stocks
  24. Preparation of DIG Labelled Probes
  25. Preparation of Mouse Muscle for Cryosectioning
  26. Preparation of Orthovanadate Stocks
  27. Preparation of RNA Sample from Cell Culture
  28. Preparation of Tail Samples (for Genotyping)
  29. Preparing an Adenoviral shRNA Clone
  30. Preparing an Agarose Gel
  31. Preparing and Sending Tissues to Histology Core
  32. Primer Design for qPCR
  33. Protein-Lipid Overlay Assay
  34. Purification of miRNA and mRNA with TRIzol
  35. QPCR - Lin Lab
  36. QPCR Analysis on ThermoCloud
  37. Quantification of Vacuolar Colocalization
  38. Quantifying Relative Expression from qRT-PCR
  39. RNA-seq Pipeline for Known Transcripts
  40. Rapamycin Stock Solution
  41. Rapid Isolation of Genomic DNA from Yeast
  42. Rate of drop analysis
  43. Restriction Enzyme Based Cloning - Ordering Primers
  44. SDS-PAGE Running Buffer
  45. SDS-PAGE Separating Gel Buffer
  46. SDS-PAGE Stacking Gel Buffer
  47. SREBF1 Genotyping Protocol
  48. SRY Genotyping
  49. Scanning and Analyzing Western Blots Using LiCor Odyssey
  50. Seahorse XF Media

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