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Glucose Tolerance Test

79 bytes added, 15:07, 20 May 2009
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==Materials (Bring Downstairs)==
*Glucometer- AccuChek Advantage*Glucose Test Strips- AccuChek Comfort Curve
*Scale
*Beaker for weighing mice
*Syringes
*10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.
*Timer
==Protocol==
#Remove food from mice for about 6h. Add do not feed tag to cages.
#Bring mice to procedure room. Put water bottle on cage.
#Weigh mice, mark tails as necessary and take fasting glucose measurement.
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).

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