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Showing below up to 96 results in range #51 to #146.

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51. How to design primer pairs for determining the presence of CrispR-cas induced mutations
52. Immobilizing a Protein on a CM5 Chip for SPR
53. Immunoprecipitation
54. Inositol Labelling of Yeast Cells
55. Installing Software to a UNIX Server
56. Isolation of Mouse Embryonic Fibroblasts (MEFs)
57. Isolation of Single Fibers for Seahorse Assays
58. Ketone Body Tolerance Test
59. Lipid Transfection
60. Lipofectamine Transfection of C2C12 Cells
61. Lipolysis Assay from Primary Adipocytes
62. Liposome Binding Assay
63. Main Page
64. Maintanence of 18C Fly Stocks
65. Mammary Gland Fat and SVF Separation
66. Matrigel Basement Membrane Matrix
67. Measurement of Glycolysis and Respiration Using Seahorse
68. Measuring Autophagy in Tissue Culture Cells
69. Measuring Cross-Sectional Area (CSA)
70. Measuring Grip Strength
71. Measuring Lifespan in Drosophila
72. Measuring Phagocytosis using pHrodo Bioparticles
73. Milk Creamatocrit
74. NADH NBT Staining
75. Oil red o stain for in vitro adipogenesis
76. Ordering Barcodes for Mice
77. Ordering Prescriptions-University of Michigan
78. Osmotic Minipump Implantation
79. PAS Staining
80. PCR Amplification of DNA (KOD Polymerase)
81. PCR Amplification with Platinum Taq High Fidelity
82. PEI Mediated Plasmid Transfection
83. Parsing XSQ Files
84. Performing Drosophila Crosses
85. Phosphate Buffered Saline
86. Phred Quality Scores
87. Preparation of Mouse Muscle for Cryosectioning
88. Preparation of Protein Lysates from Cells
89. Preparation of RNA Sample from Cell Culture
90. Preparation of Tail Samples (for Genotyping)
91. Preparing and Sending Tissues to Histology Core
92. Protein-Lipid Overlay Assay
93. Purification of GST-HA-S6K
94. Purification of GST Fusion Proteins by GSTrap
95. Purification of MBP Fusion Proteins
96. Pyruvate Tolerance Test
97. Quantification of Vacuolar Colocalization
98. Quantification of miRNA by SYBR Green qPCR
99. Quantifying Relative Expression from qRT-PCR
100. RNA-seq Pipeline for Known Transcripts
101. Rab5 FRET Assay
102. Rapamycin Injections
103. Rapamycin Sensitivity Assay of Yeast
104. Rapamycin Stock Solution
105. SHROB Genotyping
106. SOP- Chloroform
107. SOP - B-mercaptoethanol
108. SOP - BVT.2733
109. SOP - Bunsen Burners
110. SOP - Centrifuge Safety
111. SOP - Ethidium Bromide
112. SREBF1 Genotyping Protocol
113. Seahorse - Mitochondrial Stress Test
114. Sending Samples for DNA Sequencing
115. Sequence Alignment for Phylogenetic Analysis
116. Serum Total Cholesterol Assay
117. Single fiber dissociation of intact rodent muscles
118. Spill Response Procedures for Infectious agents and Recombinant DNA
119. Statistical Analysis
120. Submitting Plasmids for Sequencing
121. Surveyor Assay for Genomic DNA Mutations
122. TAP Purification of Yeast Extracts
123. TBST
124. Tamoxifen Injections for Inducible Knockouts
125. TaqMan SNP Array
126. TaqMan miRNA Assay
127. Taqman
128. Thawing Culture Cells
129. Triglyceride Assay from Tissue Culture Cells
130. Using Bioconductor To Analyse Beadarray Data (lumi)
131. Using GSEA for Gene Set Analyses of Transcriptional Data
132. Using Git
133. Using Github and Git for Code
134. Using Snakemake on the HPC Cluster
135. Using South To Migrate a Django Database
136. Using the MBNI Cluster
137. Using the echoMRI
138. Using the pH Meter
139. Verifying Kan Deletion Mutants from Genomic DNA
140. Weigh-Suckle-Weigh Milk Volume Measurement
141. Wiki new protocols
142. Writing a Paper and Uploading Code to Github Repository
143. Yeast Invertase Assay
144. Yeast Sch9 Phosphorylation Assay
145. Yeast Transfection (Small Scale)
146. Yeast Transformation

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