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- 15:23, 10 May 2018 (diff | hist) . . (+48) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads) (current)
- 15:21, 10 May 2018 (diff | hist) . . (+6) . . Chromatin Immunoprecipitation (→Prior to starting this section:)
- 15:20, 10 May 2018 (diff | hist) . . (+18) . . Chromatin Immunoprecipitation (→Reverse Crosslinks of Protein/DNA Complexes to Free DNA)
- 17:34, 10 January 2018 (diff | hist) . . (+2) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 17:33, 10 January 2018 (diff | hist) . . (+122) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 22:13, 9 January 2018 (diff | hist) . . (-8) . . Chromatin Immunoprecipitation (→Prior to starting this section:)
- 22:09, 9 January 2018 (diff | hist) . . (0) . . ChIP Elution Buffer (current)
- 22:09, 9 January 2018 (diff | hist) . . (+313) . . ChIP Elution Buffer
- 21:41, 9 January 2018 (diff | hist) . . (+6) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 21:41, 9 January 2018 (diff | hist) . . (+2) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 21:32, 9 January 2018 (diff | hist) . . (+57) . . Chromatin Immunoprecipitation (→Before You Start)
- 21:29, 9 January 2018 (diff | hist) . . (+337) . . Dilution Buffer (current)
- 20:17, 9 January 2018 (diff | hist) . . (+71) . . N ChIP Elution Buffer (Created page with "10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water.")
- 20:15, 9 January 2018 (diff | hist) . . (+79) . . N Dilution Buffer (Created page with "0.01% SDS, 1.1% Triton X- 100, 1.2mM EDTA, 16.7mM Tris-HCl, pH 8.1, 167mM NaCl.")
- 20:15, 9 January 2018 (diff | hist) . . (+22) . . Chromatin Immunoprecipitation (→Buffers and Solutions Needed)
- 20:12, 9 January 2018 (diff | hist) . . (+139) . . Chromatin Immunoprecipitation (→Buffers and Solutions Needed)
- 20:11, 9 January 2018 (diff | hist) . . (-79) . . Chromatin Immunoprecipitation (→Buffers and Solutions Needed)
- 20:09, 9 January 2018 (diff | hist) . . (+196) . . N TE Buffer (Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | Tris|| 10mM|| 150uL || 1M || Solutions Shelf |- | EDTA || 1mM || 30uL || 500mM...") (current)
- 20:07, 9 January 2018 (diff | hist) . . (+4) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 20:05, 9 January 2018 (diff | hist) . . (+402) . . N High Salt Immune Complex Wash Buffer (Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | SDS || 0.1% || 150uL || 10% || Solutions Shelf |- | Triton X-100 || 1% || 1.5mL...") (current)
- 19:59, 9 January 2018 (diff | hist) . . (+4) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:58, 9 January 2018 (diff | hist) . . (+6) . . Low Salt Immune Complex Wash Buffer (current)
- 19:54, 9 January 2018 (diff | hist) . . (+355) . . N LiCl Immune Complex Wash Buffer (Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | NP40 || 1% || 1.5mL || 1...") (current)
- 19:54, 9 January 2018 (diff | hist) . . (+4) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:53, 9 January 2018 (diff | hist) . . (+4) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:52, 9 January 2018 (diff | hist) . . (-21) . . Low Salt Immune Complex Wash Buffer
- 19:48, 9 January 2018 (diff | hist) . . (+376) . . N Low Salt Immune Complex Wash Buffer (Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | Example || Example || Ex...")
- 19:30, 9 January 2018 (diff | hist) . . (-11) . . Chromatin Immunoprecipitation (→Prior to starting this section:)
- 19:30, 9 January 2018 (diff | hist) . . (+3) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:28, 9 January 2018 (diff | hist) . . (+6) . . Chromatin Immunoprecipitation (→Reverse Crosslinks of Protein/DNA Complexes to Free DNA)
- 19:28, 9 January 2018 (diff | hist) . . (-1) . . Chromatin Immunoprecipitation (→Prior to starting this section:)
- 19:27, 9 January 2018 (diff | hist) . . (+16) . . Chromatin Immunoprecipitation (→Immunoprecipitation)
- 19:24, 9 January 2018 (diff | hist) . . (+6) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:23, 9 January 2018 (diff | hist) . . (+19) . . Chromatin Immunoprecipitation (→Crosslinking, Lysis and Shearing of DNA)
- 19:19, 9 January 2018 (diff | hist) . . (+58) . . Chromatin Immunoprecipitation (→Immunoprecipitation)
- 19:17, 9 January 2018 (diff | hist) . . (-4) . . Chromatin Immunoprecipitation
- 19:14, 9 January 2018 (diff | hist) . . (-32) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:11, 9 January 2018 (diff | hist) . . (+3,234) . . Chromatin Immunoprecipitation (→Immunoprecipitation)
- 21:43, 5 January 2018 (diff | hist) . . (+2) . . Preparation of Protein Lysates from Cells (current)
- 18:33, 15 December 2017 (diff | hist) . . (-161) . . Preparation of Protein Lysates from Cells
- 18:28, 15 December 2017 (diff | hist) . . (+1,041) . . N Preparation of Protein Lysates from Cells (Created page with "==Materials== *RIPA Buffer (see RIPA) or other Lysis buffer. Add protease inhibitors. *Cells (fresh or frozen) ==Protocol== #Cool centrifuge to 4C #If cells...")
- 16:20, 24 October 2017 (diff | hist) . . (0) . . Submitting Plasmids for Sequencing (current)
- 16:19, 24 October 2017 (diff | hist) . . (+159) . . Submitting Plasmids for Sequencing
- 19:41, 19 October 2017 (diff | hist) . . (+114) . . Cloning CRISPR-Cas Plasmids (current)
- 19:38, 19 October 2017 (diff | hist) . . (+32) . . Submitting Plasmids for Sequencing
- 19:35, 19 October 2017 (diff | hist) . . (+752) . . Submitting Plasmids for Sequencing
- 19:26, 19 October 2017 (diff | hist) . . (+975) . . N Submitting Plasmids for Sequencing (Created page with "*Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul *These should be submitted in 1.5mL tubes *Go to https://client-seqcore.brcf.med.um...")
- 15:52, 11 August 2017 (diff | hist) . . (-8) . . Western Blotting (→Protocol)
- 15:52, 8 August 2017 (diff | hist) . . (+83) . . Bradford Assay (→Protocol)
- 15:51, 8 August 2017 (diff | hist) . . (-4) . . Bradford Assay (→Protocol)
- 14:10, 4 August 2017 (diff | hist) . . (+33) . . Bradford Assay (→Materials)
- 18:56, 29 June 2017 (diff | hist) . . (+5) . . Chromatin Immunoprecipitation (→Crosslinking, Lysis and Shearing of DNA)
- 18:55, 29 June 2017 (diff | hist) . . (+116) . . Chromatin Immunoprecipitation (→Crosslinking, Lysis and Shearing of DNA)
- 19:30, 12 June 2017 (diff | hist) . . (0) . . Chromatin Immunoprecipitation
- 15:46, 28 April 2017 (diff | hist) . . (+1) . . Tamoxifen Injections for Inducible Knockouts
- 15:45, 28 April 2017 (diff | hist) . . (+85) . . Tamoxifen Injections for Inducible Knockouts
- 15:51, 27 April 2017 (diff | hist) . . (+29) . . Tamoxifen Injections for Inducible Knockouts
- 15:49, 27 April 2017 (diff | hist) . . (+2) . . Tamoxifen Injections for Inducible Knockouts
- 15:46, 27 April 2017 (diff | hist) . . (+4) . . Tamoxifen Injections for Inducible Knockouts
- 15:45, 27 April 2017 (diff | hist) . . (+219) . . Tamoxifen Injections for Inducible Knockouts
- 15:42, 27 April 2017 (diff | hist) . . (+1,325) . . Tamoxifen Injections for Inducible Knockouts
- 19:40, 2 February 2017 (diff | hist) . . (+3) . . Assessing isoproterenol-stimulated whole-body lipolysis in vivo (→Reagents Needed)
- 19:40, 2 February 2017 (diff | hist) . . (+90) . . Assessing isoproterenol-stimulated whole-body lipolysis in vivo (→Experimental protocol)
- 19:04, 31 January 2017 (diff | hist) . . (+807) . . N Preparing and Sending Tissues to Histology Core (Created page with "#Harvest tissue from animal, place into cassette and submerge in 10% neutral buffered formalin for 24-48 hrs (depending on thickness of sample). #Move cassettes into 70% ethan...")
- 21:28, 10 November 2016 (diff | hist) . . (+85) . . Spill Response Procedures for Infectious agents and Recombinant DNA (→Certification)
- 21:27, 10 November 2016 (diff | hist) . . (+85) . . SOP - Vacuum Pumps (→Training of Personnel)
- 21:27, 10 November 2016 (diff | hist) . . (+85) . . SOP - Sensitizers (→Certification)
- 21:27, 10 November 2016 (diff | hist) . . (+86) . . SOP - Irritants (→Certification)
- 21:25, 10 November 2016 (diff | hist) . . (+85) . . SOP - Compressed Gases (→Certification)
- 21:24, 10 November 2016 (diff | hist) . . (+85) . . SOP - Needle Capping (→Certification)
- 21:23, 10 November 2016 (diff | hist) . . (+85) . . SOP - Formalin and Paraformaldehyde (→Certification)
- 21:19, 10 November 2016 (diff | hist) . . (+85) . . SOP - Ethidium Bromide (→Certification)
- 21:19, 10 November 2016 (diff | hist) . . (+85) . . SOP - Electrophoresis (→Certification)
- 21:18, 10 November 2016 (diff | hist) . . (+85) . . SOP - Centrifuge Safety (→Certification)
- 21:18, 10 November 2016 (diff | hist) . . (+85) . . SOP - Carcinogens (→Certification)
- 21:18, 10 November 2016 (diff | hist) . . (+85) . . SOP - Flammables and Combustibles (→Certification)
- 21:17, 10 November 2016 (diff | hist) . . (+85) . . SOP - Bunsen Burners (→Training of Personnel)
- 21:17, 10 November 2016 (diff | hist) . . (+85) . . SOP - Bleach (→Certification)
- 21:16, 10 November 2016 (diff | hist) . . (-89) . . SOP - Cytotoxins and Antineoplastic Drugs (→MINOR CHEMICAL SPILL)
- 21:12, 10 November 2016 (diff | hist) . . (+8,730) . . N SOP - Cytotoxins and Antineoplastic Drugs (Created page with " '''Principal Investigator (PI) Approval is Required Prior to Performing this Procedure''' =Description= This standard operating procedure outlines the handling and use of c...")
- 20:57, 10 November 2016 (diff | hist) . . (+1) . . SOP - Tamoxifen (→Waste Disposal)
- 20:56, 10 November 2016 (diff | hist) . . (+10,541) . . N SOP - Tamoxifen (Created page with " '''Laboratory Director (LD) Approval is Required Prior to Performing this Procedure''' =Description= This standard operating procedure (SOP) outlines the handling and use...")
- 20:50, 10 November 2016 (diff | hist) . . (+9,629) . . N SOP - Phenol (Created page with " '''Laboratory Director (LD) Approval is Required Prior to Performing this Procedure''' =Description= This standard operating procedure (SOP) outlines the handling and use...")
- 20:44, 10 November 2016 (diff | hist) . . (+8,109) . . N SOP - Cryogenic Materials (Created page with "=Description= This standard operating procedure (SOP) outlines the handling and use of cryogenic materials. Review this document and supply the information required in order t...")
- 20:21, 10 November 2016 (diff | hist) . . (-4) . . SOP- Chloroform (→Occupational Exposure Limits (OELs):) (current)
- 20:20, 10 November 2016 (diff | hist) . . (+11,192) . . N SOP- Chloroform (Created page with "=Description= This standard operating procedure (SOP) outlines the handling and use of chloroform. Review this document and supply the information required in order to make it...")
- 19:56, 10 November 2016 (diff | hist) . . (+11,885) . . N SOP - Biosafety Cabinets (Created page with "=Description= This standard operating procedure outlines the use of biosafety cabinets. Review this document and supply the information required in order to make it specific t...")
- 19:48, 10 November 2016 (diff | hist) . . (+2) . . SOP - B-mercaptoethanol (→Spill Procedure)
- 19:46, 10 November 2016 (diff | hist) . . (+6) . . SOP - B-mercaptoethanol (→For an actual chemical exposure/injury:)
- 19:46, 10 November 2016 (diff | hist) . . (+5) . . SOP - B-mercaptoethanol (→For an actual chemical exposure/injury:)
- 19:44, 10 November 2016 (diff | hist) . . (+2) . . SOP - B-mercaptoethanol (→For an actual chemical exposure/injury:)
- 19:44, 10 November 2016 (diff | hist) . . (+5) . . SOP - B-mercaptoethanol (→Transportation and Storage)
- 19:43, 10 November 2016 (diff | hist) . . (+6) . . SOP - B-mercaptoethanol (→Work Practice Controls)
- 19:43, 10 November 2016 (diff | hist) . . (+5) . . SOP - B-mercaptoethanol (→Potential Hazards)
- 19:42, 10 November 2016 (diff | hist) . . (+7,178) . . N SOP - B-mercaptoethanol (Created page with "=Description= This standard operating procedure (SOP) outlines the handling and use of B-mercaptoethanol (BME). Review this document and supply the information required in ord...")
- 19:34, 10 November 2016 (diff | hist) . . (+87) . . SOP - Animal Anesthetics (→Certification)
- 20:16, 29 July 2016 (diff | hist) . . (+217) . . Safety and Animal Training (→Animals)
- 21:43, 10 June 2016 (diff | hist) . . (+16) . . Chromatin Immunoprecipitation for Tissue Samples (→Sonication of Tissue) (current)
- 21:42, 10 June 2016 (diff | hist) . . (-200) . . Chromatin Immunoprecipitation for Tissue Samples (→Sonication of Tissue)
- 21:42, 10 June 2016 (diff | hist) . . (+2) . . Chromatin Immunoprecipitation for Tissue Samples (→Sonication of Tissue)
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