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Revision as of 13:40, 13 July 2009 by
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Metabolic Measurements
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Cell and Tissue Culture
Splitting Cells
Differentiation of 3T3-L1 Cells
Electroporation of 3T3-L1 Adipocytes
Fugene Transfection of 293T/COS Cells
Lipofectamine Mediated Knockdown
Immunofluoresence
Colocalization
3T3-L1 Adipocyte Fractionation
Preparing Cell Lysates
Glucose Uptake Assay
Luciferase Assay
Cloning and Molecular Biology
Transformation of Bacteria
Mutagenesis
PCR Amplification of DNA
Restriction Enzyme Based Cloning
Restriction Enzyme Based Cloning - Ordering Primers
TOPO Cloning
Preparing an Agarose Gel
Cesium Chloride Preparation of DNA
Protein Analysis
Western Blotting
Surface Plasmon Resonance - Protein Lipid Interactions
Protein Quantification
Bradford Assay
Quantification by Absorbance at 280nm
Determining Percent Purity
Protein Purification
French Press
Purification of GST Fusion Proteins
Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
Transcriptional Analysis
Real Time PCR From Cell Culture
Harvesting RNA from Cells grown in monolayer
Mouse Protocols
Genotyping
Ear Tagging and Tail Clipping
DNA Preparation from Tail Clip
PCR Analysis of Tail DNA
Metabolic Measurements
Glucose Tolerance Test
Insulin Tolerance Test
Measuring Fasting Insulin
Monitoring Food Intake
Tissue Preparation
Harvesting Mouse Tissue
Preparation of Protein Lysates from Mouse Tissues
Preparation of RNA Samples from Mouse Tissues
Figure Generation and Data Management
Generation of Figures in Illustrator
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