Difference between revisions of "Glucose Tolerance Test"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (→Materials (Bring to Procedure Room)) |
Davebridges (Talk | contribs) (Added details about ipGTT) |
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#Remove food from mice for about 6h. Add do not feed tag to cages. | #Remove food from mice for about 6h. Add do not feed tag to cages. | ||
#Bring mice to procedure room. Put water bottle on cage. | #Bring mice to procedure room. Put water bottle on cage. | ||
| − | #Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized | + | #Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capillary about 2/3 full with blood. |
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | #Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||
| − | #At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | + | #At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse or the mouth if doing an oral glucose tolerance test. |
##Immobilize mouse and restrain tail with one hand | ##Immobilize mouse and restrain tail with one hand | ||
| − | ##Aim needle between the midline and the hip bone | + | ##'''For ipGTT''' Aim needle between the midline and the hip bone |
| − | ##Insert syringe (do not inject) into cavity | + | ##'''For oGTT''' Gently inert gavage needle into mouth |
| − | ##Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over | + | ##Insert syringe (do not inject) into cavity/mouth |
| + | ##'''For ipGTT:''' Pull up the syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over | ||
##Eject syringe. | ##Eject syringe. | ||
| − | #At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein | + | #At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein |
[[Category:Mouse Work]] | [[Category:Mouse Work]] | ||
[[Category:Glucose Homeostasis]] | [[Category:Glucose Homeostasis]] | ||
[[Category:Injections]] | [[Category:Injections]] | ||
Latest revision as of 17:37, 28 March 2023
SOP
Materials (Bring to Procedure Room)
- Glucometer - AccuChek Advantage
- Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [here]. collect in university hospital building, floor B2 in room B2F408 dock 5 across from central pharmacy.
- Scale
- Beaker for weighing mice
- Syringes
- 10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.
- If doing an oral glucose tolerance test need a gavage needle
- Timer
Protocol
- Remove food from mice for about 6h. Add do not feed tag to cages.
- Bring mice to procedure room. Put water bottle on cage.
- Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for Measuring Fasting Insulin by filling a heparinized capillary about 2/3 full with blood.
- Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
- At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse or the mouth if doing an oral glucose tolerance test.
- Immobilize mouse and restrain tail with one hand
- For ipGTT Aim needle between the midline and the hip bone
- For oGTT Gently inert gavage needle into mouth
- Insert syringe (do not inject) into cavity/mouth
- For ipGTT: Pull up the syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
- Eject syringe.
- At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein
