Genotyping Program

Allele	Program	Fragment Size(s)	Notes
TSC-floxed	Cre PCR	KO- 368bp, WT- 295bp	Two bands if heterozygote
Cre	Cre PCR		Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14	Cre PCR		Two bands if heterozygote
TC10b	TC10b	KO - 500bp, WT-1kb	Two bands if heterozygote
ob/ob Genotyping	ob/ob	KO - 123bp, WT-104kb, control 191 bp	Two reactions, see ob/ob Genotyping
MTM1	MTM1	Mutant-575bp, WT-714bp	Separate Reactions for WT and KO
CAP	CAP		Two bands if heterozygote, upper band for KO
Vinexin	Vinexin
GR KO	nr3c1	KO- 300bp, WT- 247bp	Two bands if heterozygote
mc4r-Cone	mc4r	KO- 540bp, WT- 400bp	Two bands if heterozygote (run primers separately)
PTG	ppp1r3c (PTG)	KO- 574bp, WT- 678bp	Two bands if heterozygote (run primers separately)
4EBP1/2	Cre PCR	1= KO- 550bp, WT- 175bp; 2= KO- 750bp, WT- 450bp	Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
GDF15	GDF15	KO- 200bp, WT- 600bp	Two bands if heterozygote (run primers separately)