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1. 3T3-L1 Adipocyte Fractionation
2. 3T3-L1 Plasma Membrane Isolation
3. Adding New Personnel to Animal Protocol
4. Adipocyte Cell Counting with ImageJ
5. Affinity Purification of Proteins - FPLC
6. Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
7. Amylose pull-down of glycogen-bound proteins
8. Analysing Data on Armis or Great Lakes using Rmd Files
9. Analysis of FRET Images
10. Antisense Oligo Knockdown in Mice
11. BDNF PCR
12. BHB Assay
13. Bradford Assay
14. CPY Assay
15. Cesium Chloride Preparation of DNA
16. ChIP Elution Buffer
17. Check Clones for Correct Mutation
18. Chromatin Immunoprecipitation for Tissue Samples
19. Collecting and Storing Mouse Serum
20. Colocalization
21. Connect to Network Drives
22. Cortisol ELISA
23. DNA Preparation from Tail Clip
24. Deparaffinizing Histology Slides
25. Designing Clones Using Benchling
26. Determining Cortisol Levels in Human Hair
27. Determining Mitochondrial Copy Number
28. Determining Percent Purity
29. Differentiation of MEFs
30. Dilution Buffer
31. Dilution Formula
32. Electroporation of 3T3-L1 Adipocytes
33. FDB Muscle Dissection from Mice
34. Fly Food Protocol
35. French Press
36. GST-EEA1 Pulldown from Yeast
37. Generation of Figures in Illustrator
38. H&E Staining
39. H&E Staining of Mouse Tissue
40. HPLC - Plumbing Setup
41. HPLC - Running a Sample or Series
42. Harvesting RNA from Cells grown in monolayer
43. High Salt Immune Complex Wash Buffer
44. How to Find a Journal Article
45. How to design primer pairs for determining the presence of CrispR-cas induced mutations
46. Immobilizing a Protein on a CM5 Chip for SPR
47. Isolation of Mouse Embryonic Fibroblasts (MEFs)
48. Isolation of Single Fibers for Seahorse Assays
49. LiCl Immune Complex Wash Buffer
50. Locating ChIP-seq peaks from ENCODE

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