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1. 3T3-L1 Adipocyte Fractionation
2. 3T3-L1 Plasma Membrane Isolation
3. Adding New Personnel to Animal Protocol
4. Adipocyte Cell Counting with ImageJ
5. Affinity Purification of Proteins - FPLC
6. Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
7. Amylose pull-down of glycogen-bound proteins
8. Analysing Data on Armis or Great Lakes using Rmd Files
9. Analysis of FRET Images
10. Antisense Oligo Knockdown in Mice
11. BDNF PCR
12. BHB Assay
13. Bradford Assay
14. CPY Assay
15. Cesium Chloride Preparation of DNA
16. ChIP Elution Buffer
17. Check Clones for Correct Mutation
18. Chromatin Immunoprecipitation for Tissue Samples
19. Collecting and Storing Mouse Serum
20. Colocalization
21. Connect to Network Drives
22. DNA Preparation from Tail Clip
23. Deparaffinizing Histology Slides
24. Designing Clones Using Benchling
25. Determining Cortisol Levels in Human Hair
26. Determining Mitochondrial Copy Number
27. Determining Percent Purity
28. Differentiation of MEFs
29. Dilution Buffer
30. Dilution Formula
31. Electroporation of 3T3-L1 Adipocytes
32. FDB Muscle Dissection from Mice
33. Fly Food Protocol
34. French Press
35. GST-EEA1 Pulldown from Yeast
36. Generation of Figures in Illustrator
37. H&E Staining
38. H&E Staining of Mouse Tissue
39. HPLC - Plumbing Setup
40. HPLC - Running a Sample or Series
41. Harvesting RNA from Cells grown in monolayer
42. High Salt Immune Complex Wash Buffer
43. How to Find a Journal Article
44. How to design primer pairs for determining the presence of CrispR-cas induced mutations
45. Immobilizing a Protein on a CM5 Chip for SPR
46. Insulin Tolerance Test
47. Isolation of Mouse Embryonic Fibroblasts (MEFs)
48. Isolation of Single Fibers for Seahorse Assays
49. LiCl Immune Complex Wash Buffer
50. Locating ChIP-seq peaks from ENCODE

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