| Allele |
Program |
Fragment Size(s) |
Notes
|
| TSC-floxed |
Cre PCR |
KO- 368bp, WT- 295bp |
Two bands if heterozygote
|
| Cre |
Cre PCR |
|
Only present if Transgene Positive (cannot detect homozygosity at transgene)
|
| Vac14 |
Cre PCR |
|
Two bands if heterozygote
|
| TC10b |
TC10b |
KO - 500bp, WT-1kb |
Two bands if heterozygote
|
| ob/ob Genotyping |
ob/ob |
KO - 123bp, WT-104kb, control 191 bp |
Two reactions, see ob/ob Genotyping
|
| MTM1 |
MTM1 |
Mutant-575bp, WT-714bp |
Separate Reactions for WT and KO
|
| CAP |
CAP |
|
Two bands if heterozygote, upper band for KO
|
| Vinexin |
Vinexin |
|
|
| GR KO |
nr3c1 |
KO- 300bp, WT- 247bp |
Two bands if heterozygote
|
| mc4r-Cone |
mc4r |
KO- 540bp, WT- 400bp |
Two bands if heterozygote (run primers separately)
|
| PTG |
ppp1r3c (PTG) |
KO- 574bp, WT- 678bp |
Two bands if heterozygote (run primers separately)
|
| 4EBP1/2 |
Cre PCR |
1=KO- 550bp, WT- 175bp; 2=KO- 750bp, WT- 450bp |
Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
|