PCR Analysis of Tail DNA: Difference between revisions
m →Protocol: Master Mix |
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| Line 13: | Line 13: | ||
#Template: 1 uL | #Template: 1 uL | ||
Master Mix | Master Mix (Per 5mL -- Make 1mL Aliquots) | ||
#10X GoTaq Buffer: | #10X GoTaq Buffer: 625uL ("Molecular Biology Stuff" box in freezer) | ||
#Primer Mix: | #Primer Mix: 625ul | ||
#dNTPs: 5uL of 10 mM ("Molecular Biology Stuff" box in freezer) | #dNTPs: 62.5uL of 10 mM ("Molecular Biology Stuff" box in freezer) | ||
#Sterile water: | #Sterile water: 3625ul | ||
#Polymerase Go-Taq: | #Polymerase Go-Taq: 15.625ul ("Molecular Biology Stuff" box in freezer) | ||
*Add Template Individually | *Add Template Individually | ||