User contributions for Mpeloqu1
1 April 2014
- 14:1314:13, 1 April 2014 diff hist +491 N BDNF PCR Created page with "'''BDNF Primers''' ---- * oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common) * oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse) * oIMR01694 --- GGG AAC TTC CTG ACT AG..." current
- 14:0314:03, 1 April 2014 diff hist +13 Main Page Added BNDF
19 February 2014
- 15:0315:03, 19 February 2014 diff hist −1 Rapamycin Injections No edit summary
1 November 2013
- 15:0915:09, 1 November 2013 diff hist +990 N Paraffin Embedding of Tissue Samples Created page with " == Protocol == #After harvesting tissue from animal, place into pathology cassette and fix in 4% formaldehyde, 4% paraformaldehyde or 4% formalin O/N #After fixation, begin d..."
- 14:5014:50, 1 November 2013 diff hist +42 Main Page →Tissue Preparation
13 September 2013
- 13:5613:56, 13 September 2013 diff hist 0 Fly Food Protocol →Cooking Insturctions current
3 September 2013
- 17:3717:37, 3 September 2013 diff hist +7 Fly Food Protocol →Cooking Insturctions
- 17:3617:36, 3 September 2013 diff hist +2,037 N Fly Food Protocol Created page with " == Quantities == '''Summer''' #900mL - Corn Syrup #202.4g - Yeast #117.2g - Soy Flour #856g - Corn Meal #67.6g - Agar #15L - Water #65mL - Propionic Acid..."
- 16:2916:29, 3 September 2013 diff hist +23 Main Page →Fly Stocks
7 August 2013
- 15:2315:23, 7 August 2013 diff hist +17 RIPA Buffer →RIPA Buffer (for 10mL lysis buffer)
- 15:2215:22, 7 August 2013 diff hist +32 RIPA Buffer →RIPA Buffer (for 10mL lysis buffer)
- 15:2115:21, 7 August 2013 diff hist 0 RIPA Buffer →RIPA Buffer (for 10mL lysis buffer)
6 August 2013
- 16:0916:09, 6 August 2013 diff hist +1 Maintenance of 18C Fly Stocks →=Maintenance current
- 16:0916:09, 6 August 2013 diff hist +397 N Maintenance of 18C Fly Stocks Created page with "==Location== Drosophila stock vials can be found in the 18C cooler of the Reiter Laboratory ==Maintenance= *Drosophila stocks are to be flipped every 3 weeks *When flipping t..."
- 16:0416:04, 6 August 2013 diff hist 0 Main Page →Fly Stocks
- 16:0216:02, 6 August 2013 diff hist +360 N Maintanence of 18C Fly Stocks Created page with "==Location== Drosophila stocks can be found in the 18C cooler of the Reiter Lab ==Maintenance== *Drosophila stocks are to be "flipped" every 3 weeks *Always flip the oldest o..." current
- 15:5515:55, 6 August 2013 diff hist +51 Main Page →Mouse/Fly Protocols
- 15:5415:54, 6 August 2013 diff hist +4 Main Page →Mouse Protocols
1 August 2013
- 14:2914:29, 1 August 2013 diff hist +102 Triglyceride Assay from Drosophila (German Method) →Protocol current
31 July 2013
- 21:2621:26, 31 July 2013 diff hist +1,305 Triglyceride Assay from Cells and Tissues →Protocol
- 21:1221:12, 31 July 2013 diff hist +412 N Triglyceride Assay from Drosophila (German Method) Created page with "==Protocol== #Use 5 female or 8 Male flies for Assay #Homogenize flies (3 min @ 40Hz) in 1mL of .05% Tween (from Tween-20 stock) #Heat samples in 70C waterbath for 5 minutes #..."
- 21:1121:11, 31 July 2013 diff hist −1,406 Triglyceride Assay from Cells and Tissues →Protocol: Creation of Protocol
- 21:0521:05, 31 July 2013 diff hist +56 m Main Page →Protein Purification
26 July 2013
- 21:2721:27, 26 July 2013 diff hist +13 m Preparation of Protein Lysates from Mouse Tissues →Protocol
- 21:2321:23, 26 July 2013 diff hist +266 m Bradford Assay →Protocol: Added Protein Lysate
- 13:5513:55, 26 July 2013 diff hist +39 PCR Analysis of Tail DNA →Protocol
25 July 2013
- 15:0015:00, 25 July 2013 diff hist +247 m PCR Analysis of Tail DNA →Protocol: Master Mix
11 July 2013
- 19:3919:39, 11 July 2013 diff hist +133 m Western Blotting →Protocol: LiCor Addition
- 19:3419:34, 11 July 2013 diff hist +2 m Transfer Buffer →10X Transfer Buffer: Changed from 10L total to 4L total current
- 19:3319:33, 11 July 2013 diff hist −6 m Western Blotting →Protocol: Changed wash times
28 June 2013
- 17:1017:10, 28 June 2013 diff hist 0 m Triglyceride Assay from Cells and Tissues →Protocol: Volume of butanol mixture
26 June 2013
- 17:1817:18, 26 June 2013 diff hist +31 m Preparation of Tail Samples (for Genotyping) →Protocol: Proteinase K
19 June 2013
- 15:2915:29, 19 June 2013 diff hist −55 m Triglyceride Assay from Cells and Tissues Deletion of unnecessary information (1/10 Glycerol Dilution)
- 15:2415:24, 19 June 2013 diff hist −466 Triglyceride Assay from Cells and Tissues updated volumes and lysis procedure