Difference between revisions of "Main Page"
From Bridges Lab Protocols
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==Cloning and Molecular Biology== | ==Cloning and Molecular Biology== | ||
Revision as of 15:43, 7 August 2009
Contents
Cell and Tissue Culture
- Splitting Cells
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Fugene Transfection of 293T/COS Cells
- Lipofectamine Mediated Knockdown
- Immunofluoresence
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Glucose Uptake Assay
- Luciferase Assay
- Inositol Labeling and Lipid Extraction
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
- Cesium Chloride Preparation of DNA
Protein Analysis
Protein Quantification
Protein Purification
- French Press
- Purification of GST Fusion Proteins
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
Transcriptional Analysis
Mouse Protocols
Genotyping
Metabolic Measurements
Tissue Preparation
- Harvesting Mouse Tissue
- Preparation of Protein Lysates from Mouse Tissues
- Preparation of RNA Samples from Mouse Tissues
